Detailed information of TA system TA154481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2892507..2893109
• Replicon: chromosome
• Accession: NZ_CP051615
• Organism: Escherichia coli O25b:H4-ST131 strain 2017_APHA

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HHM36_RS13820
• Protein ID: WP_000897302.1
• Coordinates: 2892507..2892818 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHM36_RS13825
• Protein ID: WP_000356397.1
• Coordinates: 2892819..2893109 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHM36_RS13795	2888421..2889020	+	600	WP_001296610.1	glucose-1-phosphatase	-
HHM36_RS13800	2889014..2889886	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HHM36_RS13805	2889883..2890320	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHM36_RS13810	2890365..2891306	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHM36_RS13815	2891370..2892278	-	909	WP_001385591.1	alpha/beta hydrolase	-
HHM36_RS13820	2892507..2892818	+	312	WP_000897302.1	hypothetical protein	Toxin
HHM36_RS13825	2892819..2893109	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHM36_RS13830	2893468..2893746	+	279	WP_001296612.1	hypothetical protein	-
HHM36_RS13835	2894143..2894361	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
HHM36_RS13840	2894546..2894995	-	450	WP_032140890.1	hypothetical protein	-
HHM36_RS13845	2895311..2896159	-	849	WP_001038650.1	hypothetical protein	-
HHM36_RS13850	2896449..2896691	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
HHM36_RS13855	2896873..2897802	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T154481 WP_000897302.1 NZ_CP051615:2892507-2892818 [Escherichia coli O25b:H4-ST131]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T154481 NZ_CP068091:c118443-117958 [Shigella boydii]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT154481 WP_000356397.1 NZ_CP051615:2892819-2893109 [Escherichia coli O25b:H4-ST131]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT154481 NZ_CP068091:c118715-118431 [Shigella boydii]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Structures

Toxin

• AlphaFold DB: S1P416