Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2078252..2079051 Replicon chromosome
Accession NZ_CP051615
Organism Escherichia coli O25b:H4-ST131 strain 2017_APHA

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HHM36_RS09870 Protein ID WP_000347251.1
Coordinates 2078587..2079051 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag HHM36_RS09865 Protein ID WP_001296435.1
Coordinates 2078252..2078587 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM36_RS09850 2074037..2074807 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
HHM36_RS09855 2074823..2076157 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
HHM36_RS09860 2076532..2078103 + 1572 WP_001273763.1 galactarate dehydratase -
HHM36_RS09865 2078252..2078587 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HHM36_RS09870 2078587..2079051 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HHM36_RS09875 2079106..2079915 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HHM36_RS09880 2080164..2081444 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
HHM36_RS09885 2081467..2081940 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HHM36_RS09890 2081951..2082730 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HHM36_RS09895 2082720..2083598 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HHM36_RS09900 2083616..2084050 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T154479 WP_000347251.1 NZ_CP051615:2078587-2079051 [Escherichia coli O25b:H4-ST131]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T154479 NZ_CP068091:c60549-60151 [Shigella boydii]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT154479 WP_001296435.1 NZ_CP051615:2078252-2078587 [Escherichia coli O25b:H4-ST131]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT154479 NZ_CP068091:c60776-60549 [Shigella boydii]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References