Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2036544..2037271 Replicon chromosome
Accession NZ_CP051615
Organism Escherichia coli O25b:H4-ST131 strain 2017_APHA

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HHM36_RS09655 Protein ID WP_000550189.1
Coordinates 2036957..2037271 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HHM36_RS09650 Protein ID WP_000560269.1
Coordinates 2036544..2036960 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM36_RS09640 2031904..2034255 + 2352 WP_000695432.1 alpha-glucosidase -
HHM36_RS09645 2034481..2036499 + 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HHM36_RS09650 2036544..2036960 - 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HHM36_RS09655 2036957..2037271 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HHM36_RS09660 2037556..2038692 - 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HHM36_RS09665 2038777..2039280 + 504 WP_001295542.1 M48 family metallopeptidase -
HHM36_RS09670 2039357..2040049 + 693 WP_000942551.1 vancomycin high temperature exclusion protein -
HHM36_RS09675 2040128..2041114 + 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T154478 WP_000550189.1 NZ_CP051615:c2037271-2036957 [Escherichia coli O25b:H4-ST131]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T154478 NZ_CP068091:49749-50054 [Shigella boydii]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT154478 WP_000560269.1 NZ_CP051615:c2036960-2036544 [Escherichia coli O25b:H4-ST131]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT154478 NZ_CP068091:49529-49747 [Shigella boydii]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References