Detailed information of TA system TA154463

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 112258..112497
• Replicon: plasmid pAPHA_2019
• Accession: NZ_CP051610
• Organism: Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: HHM37_RS25060
• Protein ID: WP_023144756.1
• Coordinates: 112363..112497 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	112258..112318 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHM37_RS25025	107273..107833	+	561	WP_000139341.1	fertility inhibition protein FinO	-
HHM37_RS25030	107964..108176	+	213	WP_013023861.1	hypothetical protein	-
HHM37_RS25035	108735..109127	+	393	WP_185161629.1	transposase	-
HHM37_RS25040	109161..109858	+	698	Protein_123	IS1 family transposase	-
HHM37_RS25045	109933..110283	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
HHM37_RS25050	110314..111927	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
HHM37_RS25055	112005..112291	+	287	Protein_126	DUF2726 domain-containing protein	-
-	112258..112318	-	61	NuclAT_0	-	Antitoxin
-	112258..112318	-	61	NuclAT_0	-	Antitoxin
-	112258..112318	-	61	NuclAT_0	-	Antitoxin
-	112258..112318	-	61	NuclAT_0	-	Antitoxin
HHM37_RS25060	112363..112497	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
HHM37_RS25065	112794..113048	+	255	WP_000083850.1	replication regulatory protein RepA	-
HHM37_RS25070	113285..113351	+	67	WP_185161630.1	RepA leader peptide Tap	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17	senB	1..113351	113351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T154463 WP_023144756.1 NZ_CP051610:112363-112497 [Escherichia coli O25b:H4-ST131]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T154463 NZ_CP068079:c890793-890539 [Staphylococcus carnosus]
ATTTCATTTTCTCCTGAAGTATTTGAAGATTATAAAAATTGGCAAACTCAAGATAAAAAAGTTTGGAAGAAAATCAATCA
ATTGATTAAAAGTATTGATCGTGATGGCATGTTAGATGGAATTGGTAAGCCAGAACGTTTAAAAGGTGGTTTCAGTGGTT
GGTATAGCAGACGTATCACACAAGATCACAGATTAGTATATAAAGTAACAAGTGAATCAATTTTAGCTGCTTCATGCAAG
TATCATTATCAATAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT154463 NZ_CP051610:c112318-112258 [Escherichia coli O25b:H4-ST131]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin