Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4178457..4179136 Replicon chromosome
Accession NZ_CP051609
Organism Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag HHM37_RS19880 Protein ID WP_000057523.1
Coordinates 4178457..4178759 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag HHM37_RS19885 Protein ID WP_000806442.1
Coordinates 4178795..4179136 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM37_RS19855 4173833..4175485 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
HHM37_RS19860 4175523..4176026 - 504 WP_000667000.1 hypothetical protein -
HHM37_RS19865 4176023..4176823 - 801 WP_000439798.1 hypothetical protein -
HHM37_RS19870 4176847..4177326 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
HHM37_RS19875 4177530..4178324 - 795 WP_000365147.1 TraB/GumN family protein -
HHM37_RS19880 4178457..4178759 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HHM37_RS19885 4178795..4179136 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
HHM37_RS19890 4179194..4181698 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
HHM37_RS19895 4181960..4182892 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T154456 WP_000057523.1 NZ_CP051609:4178457-4178759 [Escherichia coli O25b:H4-ST131]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T154456 NZ_CP068063:456921-457028 [Staphylococcus equorum]
GTGGTTCCAATTGTAGAAGCACTACACTTAATGTTAGGTTTCGGTACTTTCATCGTTACCTTATTAGGTTTAGTGGTTGC
AATTGTTAAATTAAGCCATAAAAAATAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT154456 WP_000806442.1 NZ_CP051609:4178795-4179136 [Escherichia coli O25b:H4-ST131]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT154456 NZ_CP068063:c457180-457018 [Staphylococcus equorum]
ATTTGATAATATTTTTATGTTGTGGTAATTTATATATAGAAAAAGGGCAACACACCATAAGTGTATCGCCCTAATGAGCC
CGTTAAAAAGACGGTGGCTGGAATTTAATAATGATTAAATAATCATCTCATCCTCGCCAAAGTTAGAGATGGTTATTTTT
TAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References