Detailed information of TA system TA154387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 284077..284665
• Replicon: chromosome
• Accession: NZ_CP051533
• Organism: Helicobacter pylori strain LIM-010

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: M3P300
• Locus tag: HG583_RS01380
• Protein ID: WP_000921941.1
• Coordinates: 284384..284665 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: -
• Locus tag: HG583_RS01375
• Protein ID: WP_202143128.1
• Coordinates: 284077..284391 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HG583_RS01355	280088..280216	+	129	WP_202143429.1	chromosome partitioning protein ParA	-
HG583_RS01360	280304..280588	+	285	WP_202143126.1	hypothetical protein	-
HG583_RS01365	280632..281806	+	1175	Protein_262	hypothetical protein	-
HG583_RS01370	281831..283741	-	1911	WP_202143127.1	relaxase/mobilization nuclease domain-containing protein	-
HG583_RS01375	284077..284391	+	315	WP_202143128.1	hypothetical protein	Antitoxin
HG583_RS01380	284384..284665	+	282	WP_000921941.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
HG583_RS01385	284915..286663	-	1749	WP_202143129.1	type IV secretory system conjugative DNA transfer family protein	-
HG583_RS01390	286664..286870	-	207	WP_202143430.1	hypothetical protein	-
HG583_RS01395	287010..287220	-	211	Protein_268	hypothetical protein	-
HG583_RS01400	287221..287508	-	288	WP_202143130.1	hypothetical protein	-
HG583_RS01405	287505..287660	-	156	Protein_270	mobilization protein	-
HG583_RS01410	287711..288382	+	672	WP_202143131.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10891.80 Da
• Isoelectric Point: 9.5726

>T154387 WP_000921941.1 NZ_CP051533:284384-284665 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRITESDVTSVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T154387 NZ_CP068035:c3948004-3947693 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12136.65 Da
• Isoelectric Point: 5.2066

>AT154387 WP_202143128.1 NZ_CP051533:284077-284391 [Helicobacter pylori]
MSNIIMDYSQYNEKQLHNFLNSIEKQLLKAEGDKNKAIKKIQECELQEQMIRQVLAQKHSQEKEPTPSLLNTIASKDDPE
CDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT154387 NZ_CP068035:c3947696-3947277 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: M3P300