Detailed information of TA system TA154370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3594521..3595274
• Replicon: chromosome
• Accession: NZ_CP051516
• Organism: Paraburkholderia aromaticivorans strain AR20-38

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: HF916_RS44350
• Protein ID: WP_168794945.1
• Coordinates: 3594521..3594802 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HF916_RS44355
• Protein ID: WP_168794946.1
• Coordinates: 3594819..3595274 (+)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF916_RS44340	3590177..3592873	+	2697	WP_168794944.1	aminopeptidase N	-
HF916_RS44345	3592968..3594331	+	1364	WP_168789116.1	IS3 family transposase	-
HF916_RS44350	3594521..3594802	+	282	WP_168794945.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HF916_RS44355	3594819..3595274	+	456	WP_168794946.1	HigA family addiction module antidote protein	Antitoxin
HF916_RS44360	3595396..3596412	+	1017	WP_168794947.1	class 1 fructose-bisphosphatase	-
HF916_RS44370	3597094..3599781	+	2688	WP_168795855.1	excinuclease ABC subunit UvrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3593495..3594331	836

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-84)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10741.38 Da
• Isoelectric Point: 9.8546

>T154370 WP_168794945.1 NZ_CP051516:3594521-3594802 [Paraburkholderia aromaticivorans]
MITTFGDKATARIFQGKFVYSLPLHMQQLARRKLFMIDAAESIRNLHAPPGNRLEALQGQRSGQWSIRINAQWRICFHFV
DGEALNVEIVDYH

Nucleotide

• Length: 282 bp

>T154370 NZ_CP068034:c1070537-1070133 [Pseudomonas syringae]
ATGCTCAAGTACATGCTTGACACCAATATTTGCATTTTCACTATAAAGAACAAGCCAACTTCGGTGCGTGAAGCCTTCAA
CCTCCATCACGGACAGCTATGTATCAGCGCGATCACGTTGATGGAGCTTGTCTACGGCGCAGAAAAGTCTTTGAGCCCCG
AAAGAAACCTTGCCGTTGTAGAGGGTTTCACAGCGCGTCTTGAAGTGCTCCCTTACGACAGCGACGCTGCGGCTCATACC
GGCATGATCAGAGCCGAACTGGCTAGATCGGGCACACCGATCGGCCCGTACGATCAAATGATTGCAGGACACGCCCGCTC
TCTAGGCCTGGTGGTGATCACTAATAATCAGCGTGAATTCCGACGCGTCGAGGGGCTACGAGTAGAAGACTGGGTAAGCC
AGTAA

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 16392.88 Da
• Isoelectric Point: 9.9940

>AT154370 WP_168794946.1 NZ_CP051516:3594819-3595274 [Paraburkholderia aromaticivorans]
MVIKRSDLGSIDFSDIDTGDSIPEIHPGEILRSEFLEPLGMSVNALALALRVPAPRINDVVRGKRAISADTALRLERYFG
ASAQFWLNLQIAYDLRVATAAAGEQIEREIEPMPKAERPKMPKVSAEHARAAALAASVSGKLTASKGRRKT

Nucleotide

• Length: 456 bp

>AT154370 NZ_CP068034:c1070767-1070537 [Pseudomonas syringae]
ATGGAACAATCCACAGTCTTCAAAAGCAATCGAAGCCAGGCTGTCCGACTGCCAAAAGCGGTGGCCTTGCCCGACGACGT
GAAACGTGTCGATGTCGTAGCCGTGGGCAGAACCCGCATTATTTCCCCCGCTGGTGAAATGTGGAACAGCTGGTTCGATG
GTGAAAGTGTCAGTGATGATTTCATGGCAGAGCGCGGACAACCAGCCGAACAGCAGCGCGAATCGCTCTGA