Detailed information of TA system TA154351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1446036..1446624
• Replicon: chromosome
• Accession: NZ_CP051507
• Organism: Helicobacter pylori strain SHIM-002

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: M3P300
• Locus tag: HG560_RS06880
• Protein ID: WP_000921941.1
• Coordinates: 1446343..1446624 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: -
• Locus tag: HG560_RS06875
• Protein ID: WP_000065283.1
• Coordinates: 1446036..1446350 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HG560_RS06855	1441597..1442253	+	657	WP_000587367.1	ParA family protein	-
HG560_RS06860	1442338..1442607	+	270	WP_000394629.1	hypothetical protein	-
HG560_RS06865	1442639..1443823	+	1185	WP_000665505.1	hypothetical protein	-
HG560_RS06870	1443848..1445755	-	1908	WP_001162713.1	relaxase/mobilization nuclease domain-containing protein	-
HG560_RS06875	1446036..1446350	+	315	WP_000065283.1	hypothetical protein	Antitoxin
HG560_RS06880	1446343..1446624	+	282	WP_000921941.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
HG560_RS06885	1446899..1448626	-	1728	WP_202170283.1	type IV secretory system conjugative DNA transfer family protein	-
HG560_RS06890	1448627..1449184	-	558	WP_001167785.1	hypothetical protein	-
HG560_RS06895	1449185..1449475	-	291	WP_000159821.1	hypothetical protein	-
HG560_RS06900	1449472..1449927	-	456	Protein_1337	mobilization protein	-
HG560_RS06905	1449924..1450865	-	942	WP_000625127.1	Flp pilus assembly complex ATPase component TadA	-
HG560_RS06910	1450865..1451164	-	300	WP_000660135.1	hypothetical protein	-
HG560_RS06915	1451161..1451424	-	264	WP_000564645.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	pflA / babA/hopS / rfaJ / fliQ / fliI	1270329..1486006	215677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10891.80 Da
• Isoelectric Point: 9.5726

>T154351 WP_000921941.1 NZ_CP051507:1446343-1446624 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRITESDVTSVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T154351 NZ_CP068023:c2820367-2820107 [Pseudoalteromonas sp. GCY]
ATGAGTAGTTTATTGGCGTGGACTGATGACGCTTGGGATGATTACCTGTATTGGCAAACTCAAGAAAAGAAAACACTTAA
GCGCATCAATAAACTACTCAATGATGTTAAGCGCTCTCCATTTGAGGGCATCGGGAAACCTGAGGCGTTGAAAGAGAATC
TATCTGGTTTTTGGTCTCGTCGTATTGATGATACTAACAGACTTGTTTACGCAGTTGATGATCAAGCGATAACGATAATT
TCGTGTCGTTACCACTACTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12153.71 Da
• Isoelectric Point: 6.4659

>AT154351 WP_000065283.1 NZ_CP051507:1446036-1446350 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNAIEKQLLKAEGDKKKAIKKIQECELQEQMIRQVLAQKHSQEKEPTQSLLNTIASKDNPE
CDVSFGDFNDFLQIAKQERERRNA

Nucleotide

• Length: 315 bp

>AT154351 NZ_CP068023:c2820614-2820360 [Pseudoalteromonas sp. GCY]
ATGAAAATCGTATCTTTTACTGAAGCTAGAAATAGTCTCAAAGCTGTTTTAGACGGTGTAGTTAATGATGCTGATACAAC
AGTTATAACACGGCGTGACTCTGAGGATGCAGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAAACAGTGCATT
TACTACGTTCTCCTCAAAACGCTGAACACCTAAACCGTTCAATAGCACAGTACCGCGCTGGTAAAACAACAACGCGAGAG
TTAATTGATGAGTAG

Structures

Toxin

• AlphaFold DB: M3P300