Detailed information of TA system TA154348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1446046..1446634
• Replicon: chromosome
• Accession: NZ_CP051505
• Organism: Helicobacter pylori strain SHIM-010

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: M3P300
• Locus tag: HG562_RS06880
• Protein ID: WP_000921941.1
• Coordinates: 1446353..1446634 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: -
• Locus tag: HG562_RS06875
• Protein ID: WP_000065283.1
• Coordinates: 1446046..1446360 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HG562_RS06855	1441607..1442263	+	657	WP_000587367.1	ParA family protein	-
HG562_RS06860	1442348..1442617	+	270	WP_000394629.1	hypothetical protein	-
HG562_RS06865	1442649..1443833	+	1185	WP_000665505.1	hypothetical protein	-
HG562_RS06870	1443858..1445765	-	1908	WP_001162713.1	relaxase/mobilization nuclease domain-containing protein	-
HG562_RS06875	1446046..1446360	+	315	WP_000065283.1	hypothetical protein	Antitoxin
HG562_RS06880	1446353..1446634	+	282	WP_000921941.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
HG562_RS06885	1446909..1448636	-	1728	WP_202170283.1	type IV secretory system conjugative DNA transfer family protein	-
HG562_RS06890	1448637..1449194	-	558	WP_001167785.1	hypothetical protein	-
HG562_RS06895	1449195..1449485	-	291	WP_000159821.1	hypothetical protein	-
HG562_RS06900	1449482..1449937	-	456	Protein_1337	mobilization protein	-
HG562_RS06905	1449934..1450875	-	942	WP_000625127.1	Flp pilus assembly complex ATPase component TadA	-
HG562_RS06910	1450875..1451174	-	300	WP_000660135.1	hypothetical protein	-
HG562_RS06915	1451171..1451434	-	264	WP_000564645.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	pflA / babA/hopS / rfaJ / fliQ / fliI	1270339..1486044	215705

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10891.80 Da
• Isoelectric Point: 9.5726

>T154348 WP_000921941.1 NZ_CP051505:1446353-1446634 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRITESDVTSVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T154348 NZ_CP068022:756373-756660 [Pseudoalteromonas sp. GCY]
ATGAAAGTGGTTTGGTCTCCATTAGCGCTTCAGAAGCTAGGCGATGCAGCTGAATTTATTTCTTTGGATAATCCATCTGC
CGCAGAAAAGTGGGTGAACGAAGTTTTTGATAAAACTGAACTACTTGGAAGTATGCCTGAAATGGGGCGCTTTGTTCCTG
AAATGCCCCATACAAATTATCGCGAAATCATTTTTGGTCACTATCGTATTATCTATAGCTTAAGTCATGAGATCCGCGTA
CTAACAGTGCGTAATTGTCGTCAAATGTTGTCGGAAGATGACCTGTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12153.71 Da
• Isoelectric Point: 6.4659

>AT154348 WP_000065283.1 NZ_CP051505:1446046-1446360 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNAIEKQLLKAEGDKKKAIKKIQECELQEQMIRQVLAQKHSQEKEPTQSLLNTIASKDNPE
CDVSFGDFNDFLQIAKQERERRNA

Nucleotide

• Length: 315 bp

>AT154348 NZ_CP068022:756095-756376 [Pseudoalteromonas sp. GCY]
ATGAGTCGTATTCATTTCGATCAAGATATTCAGCCGTTATCTGAATTTCGTGCTGGTGTAGCTTCTTACATCAAACAAAT
CAATGAGACTCGCCGTCCATTGGTAATCACACAACATGGTAAAGGTGTAGCTGTTGTTCTCGATGTTGCAGAGTATGAAG
CAATGCAAGAGAAAATAGAATTACTAGAAGAAATGCGCACCGCTGAAGCTCAATTAGCTTCAGGCCTTGGTGTTTCCAAT
GATGATGCTCGTACTCAAGTACTGGGGCGCATTAAGAAATGA

Structures

Toxin

• AlphaFold DB: M3P300