Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 169522..170249 Replicon plasmid pM297-1.1
Accession NZ_CP051491
Organism Klebsiella pneumoniae strain M297-1

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag HGK25_RS26595 Protein ID WP_004118600.1
Coordinates 169522..169833 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag HGK25_RS26600 Protein ID WP_000990392.1
Coordinates 169830..170249 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGK25_RS26575 (HGK25_27120) 166036..166755 + 720 WP_001102107.1 (Fe-S)-binding protein -
HGK25_RS26580 (HGK25_27125) 166766..168193 + 1428 WP_000023895.1 LutB/LldF family L-lactate oxidation iron-sulfur protein -
HGK25_RS26585 (HGK25_27130) 168186..168881 + 696 WP_004118595.1 lactate utilization protein C -
HGK25_RS26590 (HGK25_27135) 168931..169311 - 381 Protein_180 gluconate permease -
HGK25_RS26595 (HGK25_27140) 169522..169833 + 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
HGK25_RS26600 (HGK25_27145) 169830..170249 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
HGK25_RS26605 (HGK25_27150) 170286..171488 - 1203 WP_003031541.1 hypothetical protein -
HGK25_RS26610 (HGK25_27155) 171481..171810 - 330 WP_004118613.1 hypothetical protein -
HGK25_RS26615 (HGK25_27160) 171807..172466 - 660 WP_000078540.1 hypothetical protein -
HGK25_RS28535 (HGK25_27165) 172890..173246 - 357 WP_077253206.1 Ref family recombination enhancement nuclease -
HGK25_RS28540 (HGK25_27170) 173366..173506 - 141 WP_004118614.1 hypothetical protein -
HGK25_RS26625 (HGK25_27175) 173799..174152 + 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
HGK25_RS26630 (HGK25_27180) 174200..174562 + 363 WP_020324593.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3'')-Ib / aph(6)-Id / blaCTX-M-14 / blaLAP-2 / qnrS1 - 1..222864 222864


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T154324 WP_004118600.1 NZ_CP051491:169522-169833 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T154324 NZ_CP068015:800714-801124 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT154324 WP_000990392.1 NZ_CP051491:169830-170249 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT154324 NZ_CP068015:800467-800733 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References