Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2061455..2062052 Replicon chromosome
Accession NZ_CP051490
Organism Klebsiella pneumoniae strain M297-1

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HGK25_RS09875 Protein ID WP_004142563.1
Coordinates 2061455..2061772 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HGK25_RS09880 Protein ID WP_004142561.1
Coordinates 2061765..2062052 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGK25_RS09860 (HGK25_10075) 2057158..2058585 - 1428 WP_004176980.1 MFS transporter -
HGK25_RS09865 (HGK25_10080) 2058694..2059560 + 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
HGK25_RS09870 (HGK25_10085) 2060227..2061270 + 1044 WP_032419264.1 DUF2157 domain-containing protein -
HGK25_RS09875 (HGK25_10090) 2061455..2061772 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HGK25_RS09880 (HGK25_10095) 2061765..2062052 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HGK25_RS09885 (HGK25_10100) 2062317..2062970 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
HGK25_RS09890 (HGK25_10105) 2063090..2063458 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
HGK25_RS09895 (HGK25_10110) 2063458..2064039 - 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
HGK25_RS09900 (HGK25_10115) 2064218..2065333 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
HGK25_RS09905 (HGK25_10120) 2065364..2065705 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
HGK25_RS09910 (HGK25_10125) 2065723..2065971 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T154313 WP_004142563.1 NZ_CP051490:2061455-2061772 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T154313 NZ_CP067994:c3646551-3646153 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT154313 WP_004142561.1 NZ_CP051490:2061765-2062052 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT154313 NZ_CP067994:c3646847-3646554 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References