Detailed information of TA system TA154227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2380453..2380970
• Replicon: chromosome
• Accession: NZ_CP051479
• Organism: Staphylococcus aureus strain NGA76

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: HF085_RS11655
• Protein ID: WP_000113262.1
• Coordinates: 2380704..2380970 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: HF085_RS11650
• Protein ID: WP_000584499.1
• Coordinates: 2380453..2380704 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF085_RS11620 (2376151)	2376151..2376870	+	720	WP_000479576.1	amino acid ABC transporter permease	-
HF085_RS11625 (2376867)	2376867..2377598	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
HF085_RS11630 (2377695)	2377695..2378945	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
HF085_RS11635 (2379200)	2379200..2379562	+	363	WP_000819840.1	DUF4467 domain-containing protein	-
HF085_RS11640 (2379581)	2379581..2380180	+	600	WP_000162810.1	DsbA family protein	-
HF085_RS11645 (2380208)	2380208..2380288	+	81	WP_223197974.1	hypothetical protein	-
HF085_RS11650 (2380453)	2380453..2380704	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HF085_RS11655 (2380704)	2380704..2380970	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
HF085_RS11660 (2381005)	2381005..2381076	+	72	Protein_2267	hypothetical protein	-
HF085_RS11665 (2381265)	2381265..2382818	+	1554	WP_000727769.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
HF085_RS11670 (2383004)	2383004..2383471	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
HF085_RS11675 (2383555)	2383555..2383740	+	186	WP_000230293.1	hypothetical protein	-
HF085_RS11680 (2383956)	2383956..2384780	+	825	WP_000594629.1	formate/nitrite transporter family protein	-
HF085_RS11685 (2385014)	2385014..2385550	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T154227 WP_000113262.1 NZ_CP051479:2380704-2380970 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T154227 NZ_CP067412:c5298521-5298252 [Pseudomonas sp. HS6]
ATGCAGGTTGAGTGGCAGCCCGAAGCTCGGGCTGAACTCCTGCACATTCTTGACTACATCAGTGACCGTAATTTCAAAGC
GGCTAACAAGCTTTCAAACGCTATTGAAGCCGCCACGATTATCGTTGCCAAACATCCACACCTCTATCGAGTGGGGCGAA
CTCCCGGCACTCGTGAGATGGTGGTACATCCCAACTACCTGCTCATTTACCAAGCGGTGGGCCGGATAAATATTCTGGGC
GTTGTGCACGCACGTCAGGAATATCTGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT154227 WP_000584499.1 NZ_CP051479:2380453-2380704 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT154227 NZ_CP067412:c5298717-5298511 [Pseudomonas sp. HS6]
ATGATCGACCCGTCTTTGATCGACCCACTGTGCCCGGTTGTTTCGGATCTTGGTCCCGAGGAGCAGGCTGCGAGTTATGA
CCGTTGGTTGATCGCCAAAATACAAGCCTCCATGAATGACCCACGTCCGAGCATTCCTAATGATCAGGTGATGGCCGAAA
TGCATGCCCTGATGGAAGCAAAGCGTAAAAAATACGATGCAGGTTGA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60