Detailed information of TA system TA154157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2649714..2650408
• Replicon: chromosome
• Accession: NZ_CP051458
• Organism: Dickeya solani strain IFB0231

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: HAT89_RS11670
• Protein ID: WP_026594479.1
• Coordinates: 2649714..2650112 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: A0A2K8VUD1
• Locus tag: HAT89_RS11675
• Protein ID: WP_022633740.1
• Coordinates: 2650115..2650408 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAT89_RS11640	2644894..2645928	-	1035	WP_022633732.1	harpin HrpZ family protein	-
HAT89_RS11645	2646089..2646499	-	411	WP_022633733.1	hypothetical protein	-
HAT89_RS11650	2646496..2646684	-	189	WP_022633734.1	hypothetical protein	-
HAT89_RS11655	2646719..2648788	-	2070	WP_023637836.1	type III secretion system outer membrane ring subunit SctC	-
HAT89_RS11660	2648781..2649215	-	435	WP_022633737.1	hypothetical protein	-
HAT89_RS11665	2649202..2649429	-	228	WP_022633738.1	type III secretion protein HrpF	-
HAT89_RS11670	2649714..2650112	-	399	WP_026594479.1	type II toxin-antitoxin system YafO family toxin	Toxin
HAT89_RS11675	2650115..2650408	-	294	WP_022633740.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HAT89_RS11680	2650626..2651675	-	1050	WP_022633741.1	hypothetical protein	-
HAT89_RS11685	2652215..2653375	+	1161	WP_022633742.1	alpha/beta fold hydrolase	-
HAT89_RS11690	2653386..2654699	-	1314	WP_022633743.1	lytic murein transglycosylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2649202..2659130	9928

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15230.56 Da
• Isoelectric Point: 8.4931

>T154157 WP_026594479.1 NZ_CP051458:c2650112-2649714 [Dickeya solani]
IRIFKSTLIRQQLSQQELDDLVADFHAYKQNGVLPDTFGRDAPYDDDRTYPLVKAEQVAHIHLADADAPFPRFLRQFKRT
SDRAHLVYCQGAMDPEACLLIIILKPEAHKMARNNNNMHKIGMMAAAFRMKY

Nucleotide

• Length: 399 bp

>T154157 NZ_CP067372:c4673781-4673440 [Klebsiella pneumoniae]
ATGAAAACCATACCTGCAACAACTCAGCGGGCGGCGAAGTCGTGCCCGTCGCCCGTGGCTTTCTGGCAGATGCCACTTAC
CCGTCTGCTGGAGCAGCATTATGGCCTCACCCTGAATGACACGCCGTTCAGCGATGAAACCGCTATTCAGGAACATATCA
ACGCAGGCATCACACTGGTTGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTT
AACTGTCAGGAGCAGTCCCCTTATCTGGGTGCAGTGGATATTTTGCGGGCGCGGCAGGTAACCGGCCTGTTGCGGCAAAG
CCCTCATCCTTCGATACGCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10934.48 Da
• Isoelectric Point: 5.2082

>AT154157 WP_022633740.1 NZ_CP051458:c2650408-2650115 [Dickeya solani]
MERILAEKSINITELRKNPAKYFIDEPVAVLSNNRPAGYMVSAKVFEELIDLLEAKQETVHTVARFRPTAERLRALADSG
QKLLNDASGKDLTEFTE

Nucleotide

• Length: 294 bp

>AT154157 NZ_CP067372:c4674132-4673806 [Klebsiella pneumoniae]
ATGCCACTGACTGATGCCATACAGTGGGGATTAAAGCGAAGTTTCACACCGAATTTTGGCGCAAGGCTGGTACAGGAAGG
CAACCGACTGCACTACCTGGCTGACCGGGCCAATATCACCGGTAAATTTGTTGATGCCGAATGTCAGAAGCTGGAAGAAG
CATTCCCGCATTTTATCCGCCAGATGGAACTGATGCTGAGCACTGGCGAACTTAATCCCCGTCATGCGCACAGCGTCACT
CTCTACCACAACGCTTTCACCTGTGAAGCCGATACTCTCGGCAGTTGTGGTTACGTCTACATCGTTATTTATCCCACTCA
ACGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2K8VUD1