Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ(toxin)
Location 1018304..1018892 Replicon chromosome
Accession NZ_CP051435
Organism Helicobacter pylori strain ASHA-005

Toxin (Protein)


Gene name TfiT Uniprot ID J0ILK7
Locus tag HGK50_RS04845 Protein ID WP_000921935.1
Coordinates 1018304..1018585 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name TfiA Uniprot ID G2MA82
Locus tag HGK50_RS04850 Protein ID WP_000065286.1
Coordinates 1018578..1018892 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGK50_RS04810 1013497..1013780 + 284 Protein_938 hypothetical protein -
HGK50_RS04815 1013762..1014063 + 302 Protein_939 hypothetical protein -
HGK50_RS04820 1014063..1015004 + 942 Protein_940 Flp pilus assembly complex ATPase component TadA -
HGK50_RS04825 1015001..1015456 + 456 WP_202135313.1 mobilization protein -
HGK50_RS04830 1015453..1015743 + 291 WP_202135314.1 hypothetical protein -
HGK50_RS04835 1015744..1016301 + 558 WP_202135315.1 hypothetical protein -
HGK50_RS04840 1016302..1018029 + 1728 WP_202135316.1 type IV secretory system conjugative DNA transfer family protein -
HGK50_RS04845 1018304..1018585 - 282 WP_000921935.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
HGK50_RS04850 1018578..1018892 - 315 WP_000065286.1 hypothetical protein Antitoxin
HGK50_RS04855 1019184..1019339 + 156 WP_202135317.1 relaxase -
HGK50_RS04860 1019356..1019982 + 627 WP_202135318.1 hypothetical protein -
HGK50_RS04865 1020039..1020968 - 930 WP_202135319.1 hypothetical protein -
HGK50_RS04870 1021202..1022179 + 978 WP_202135320.1 HipA domain-containing protein -
HGK50_RS04875 1022351..1022725 + 375 Protein_951 CAAX protease -
HGK50_RS04880 1022747..1023034 + 288 WP_202135321.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 989176..1048086 58910


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10861.77 Da        Isoelectric Point: 9.5726

>T154122 WP_000921935.1 NZ_CP051435:c1018585-1018304 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRITESDVASVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Download         Length: 282 bp

>T154122 NZ_CP067343:8248-8502 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12140.62 Da        Isoelectric Point: 5.2248

>AT154122 WP_000065286.1 NZ_CP051435:c1018892-1018578 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNAIEKQLLKAEGDKNKAIKKIQECELQEQMIRQVLAQKHSQEKEPTQSLLNTIASKDDPE
CDVSFGDFNDFLQIAKQERERRNA

Download         Length: 315 bp

>AT154122 NZ_CP067343:8499-8741 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L0PNN7

Antitoxin

Source ID Structure
AlphaFold DB G2MA82

References