Detailed information of TA system TA154032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 44587..45341
• Replicon: plasmid p20723-1
• Accession: NZ_CP051374
• Organism: Salmonella enterica subsp. enterica serovar Newport strain CVM 24374

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: HFS12_RS23410
• Protein ID: WP_000558166.1
• Coordinates: 45030..45341 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HFS12_RS23405
• Protein ID: WP_001259011.1
• Coordinates: 44587..45033 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFS12_RS23375	39763..40659	+	897	WP_001520529.1	sugar kinase	-
HFS12_RS23380	40693..41496	+	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
HFS12_RS23385	41687..42286	+	600	WP_000965695.1	glucose-1-phosphatase	-
HFS12_RS23390	42280..43152	+	873	WP_000921423.1	virulence factor BrkB family protein	-
HFS12_RS23395	43149..43586	+	438	WP_000560969.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HFS12_RS23400	43631..44572	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
HFS12_RS23405	44587..45033	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
HFS12_RS23410	45030..45341	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
HFS12_RS23415	45427..46356	-	930	WP_001127704.1	alpha/beta hydrolase	-
HFS12_RS23420	46574..46885	+	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
HFS12_RS23425	46886..47176	+	291	WP_000362050.1	DNA-binding transcriptional regulator	-
HFS12_RS23430	47223..48152	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
HFS12_RS23435	48149..48784	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
HFS12_RS23440	48781..49683	-	903	WP_000331364.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..194325	194325

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T154032 WP_000558166.1 NZ_CP051374:c45341-45030 [Salmonella enterica subsp. enterica serovar Newport]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T154032 NZ_CP067303:c2435042-2434939 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT154032 WP_001259011.1 NZ_CP051374:c45033-44587 [Salmonella enterica subsp. enterica serovar Newport]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT154032 NZ_CP067303:2434901-2435172 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A0R9ME90