Detailed information of TA system TA154023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1943401..1943963
• Replicon: chromosome
• Accession: NZ_CP051368
• Organism: Salmonella enterica subsp. houtenae serovar 43:z4 strain CVM 24399

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A727YRU0
• Locus tag: HFS24_RS09240
• Protein ID: WP_000597305.1
• Coordinates: 1943685..1943963 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HFS24_RS09235
• Protein ID: WP_071624889.1
• Coordinates: 1943401..1943685 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFS24_RS09200 (1938741)	1938741..1939397	-	657	WP_000100265.1	carbon-nitrogen hydrolase family protein	-
HFS24_RS09205 (1939504)	1939504..1939734	-	231	WP_000856224.1	DNA polymerase III subunit theta	-
HFS24_RS09210 (1939872)	1939872..1940246	+	375	WP_001664129.1	CopC domain-containing protein YobA	-
HFS24_RS09215 (1940247)	1940247..1941122	+	876	WP_002953395.1	copper homeostasis membrane protein CopD	-
HFS24_RS09220 (1941139)	1941139..1941489	+	351	WP_000746717.1	YebY family protein	-
HFS24_RS09225 (1941856)	1941856..1942251	-	396	Protein_1816	tyrosine-type recombinase/integrase	-
HFS24_RS23345 (1942526)	1942526..1943242	+	717	WP_258169247.1	DUF1076 domain-containing protein	-
HFS24_RS09235 (1943401)	1943401..1943685	-	285	WP_071624889.1	HigA family addiction module antitoxin	Antitoxin
HFS24_RS09240 (1943685)	1943685..1943963	-	279	WP_000597305.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HFS24_RS09245 (1944215)	1944215..1944475	+	261	WP_219827419.1	hypothetical protein	-
HFS24_RS09250 (1944546)	1944546..1945229	-	684	WP_219827315.1	PipA/GogA/GtgA family type III secretion system effector	-
HFS24_RS09255 (1945654)	1945654..1946484	+	831	WP_258169248.1	hypothetical protein	-
HFS24_RS09260 (1946487)	1946487..1947018	+	532	Protein_1823	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1919752..1946921	27169

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-63)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10737.17 Da
• Isoelectric Point: 8.4694

>T154023 WP_000597305.1 NZ_CP051368:c1943963-1943685 [Salmonella enterica subsp. houtenae serovar 43:z4]
MIKSFRHKGLNKFYQKGDSSGLEQSLVPRLKNRLSMLDAAKTIDFLNQQGYDLHELKGDRAETWSIKVSGNWRLTFIFEN
GDVYVLNLEDYH

Nucleotide

• Length: 279 bp

>T154023 NZ_CP067303:c852331-852053 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10214.76 Da
• Isoelectric Point: 5.6051

>AT154023 WP_071624889.1 NZ_CP051368:c1943685-1943401 [Salmonella enterica subsp. houtenae serovar 43:z4]
MAMFDPAYPGEIIAEILEDQGVSLRQFAVAMNIAPSTASRILSGKVNITPEMAVKLAIVLGGDAESWLNLQNNYSLSQAR
KTVDITHVHRLSFA

Nucleotide

• Length: 285 bp

>AT154023 NZ_CP067303:c852594-852334 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A727YRU0