Detailed information of TA system TA154005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 103129..103786
• Replicon: plasmid p28322-2
• Accession: NZ_CP051360
• Organism: Salmonella enterica subsp. enterica serovar Heidelberg strain CVM 28322

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: HFS63_RS25310
• Protein ID: WP_000270043.1
• Coordinates: 103436..103786 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HFS63_RS25305
• Protein ID: WP_000124640.1
• Coordinates: 103129..103431 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFS63_RS25260	98754..99182	+	429	WP_000591074.1	hypothetical protein	-
HFS63_RS25265	99239..99598	+	360	WP_000422768.1	hypothetical protein	-
HFS63_RS25270	99598..100044	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
HFS63_RS25275	100041..100559	+	519	WP_000210756.1	nitrite reductase	-
HFS63_RS25280	100559..100789	+	231	WP_000972663.1	hypothetical protein	-
HFS63_RS25285	100776..101633	+	858	WP_001167032.1	hypothetical protein	-
HFS63_RS25290	101864..102391	+	528	WP_001236377.1	thermonuclease family protein	-
HFS63_RS25295	102449..102721	+	273	WP_001043047.1	HU family DNA-binding protein	-
HFS63_RS25300	102809..103102	+	294	WP_001239997.1	chromosome segregation protein ParM	-
HFS63_RS25305	103129..103431	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
HFS63_RS25310	103436..103786	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HFS63_RS25315	103949..104497	+	549	WP_001061574.1	transcriptional regulator	-
HFS63_RS25320	104838..105032	+	195	WP_000343597.1	hypothetical protein	-
HFS63_RS25325	105043..105414	+	372	WP_000516916.1	hypothetical protein	-
HFS63_RS25330	105407..105877	+	471	WP_001281821.1	hypothetical protein	-
HFS63_RS25335	105892..106227	-	336	WP_000683476.1	hypothetical protein	-
HFS63_RS25340	106324..106812	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
HFS63_RS25345	106815..107312	+	498	WP_000062185.1	hypothetical protein	-
HFS63_RS25350	107527..108231	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HFS63_RS25355	108538..108717	+	180	Protein_131	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..167068	167068

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 17237.68 Da
• Isoelectric Point: 5.5500

>T154005 WP_000270043.1 NZ_CP051360:c103786-103436 [Salmonella enterica subsp. enterica serovar Heidelberg]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 351 bp

>T154005 NZ_CP067299:2035006-2035182 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 14882.85 Da
• Isoelectric Point: 5.5500

>AT154005 WP_000124640.1 NZ_CP051360:c103431-103129 [Salmonella enterica subsp. enterica serovar Heidelberg]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 303 bp

>AT154005 NZ_CP067299:2035228-2035644 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9