Detailed information of TA system TA153995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 18368..19025
• Replicon: plasmid p35009
• Accession: NZ_CP051316
• Organism: Salmonella enterica subsp. enterica serovar Uganda strain CVM 35009

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: HFU03_RS23400
• Protein ID: WP_000270043.1
• Coordinates: 18675..19025 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HFU03_RS23395
• Protein ID: WP_000124640.1
• Coordinates: 18368..18670 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFU03_RS23350	13993..14421	+	429	WP_000591074.1	hypothetical protein	-
HFU03_RS23355	14478..14837	+	360	WP_000422768.1	hypothetical protein	-
HFU03_RS23360	14837..15283	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
HFU03_RS23365	15280..15798	+	519	WP_000210756.1	nitrite reductase	-
HFU03_RS23370	15798..16028	+	231	WP_000972663.1	hypothetical protein	-
HFU03_RS23375	16015..16872	+	858	WP_001167032.1	hypothetical protein	-
HFU03_RS23380	17103..17630	+	528	WP_001236377.1	thermonuclease family protein	-
HFU03_RS23385	17688..17960	+	273	WP_001043047.1	HU family DNA-binding protein	-
HFU03_RS23390	18048..18341	+	294	WP_001239997.1	chromosome segregation protein ParM	-
HFU03_RS23395	18368..18670	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
HFU03_RS23400	18675..19025	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HFU03_RS23405	19188..19736	+	549	WP_001061574.1	transcriptional regulator	-
HFU03_RS23410	20077..20271	+	195	WP_000343597.1	hypothetical protein	-
HFU03_RS23415	20282..20653	+	372	WP_000516916.1	hypothetical protein	-
HFU03_RS23420	20646..21116	+	471	WP_001281821.1	hypothetical protein	-
HFU03_RS23425	21131..21466	-	336	WP_000683476.1	hypothetical protein	-
HFU03_RS23430	21563..22051	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
HFU03_RS23435	22054..22551	+	498	WP_000062185.1	hypothetical protein	-
HFU03_RS23440	22766..23470	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HFU03_RS23445	23535..23714	-	180	Protein_39	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..170832	170832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin MqsA/HigA-2 (6-70)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 17237.68 Da
• Isoelectric Point: 5.5500

>T153995 WP_000270043.1 NZ_CP051316:c19025-18675 [Salmonella enterica subsp. enterica serovar Uganda]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 351 bp

>T153995 NZ_CP067270:33122-33382 [Escherichia coli]
ATGAGAAAAAACAAAGGAGATATTACCTACTATCTTGAGGAAAACGCAGGTGTATACCATTTCGTAAAAAAGATTAAGAC
TCGTTCAAAACTCATGACCGAAGGTAAAAGTAAAATTACTAAAAAAATGTTAAGTGAGTTTTATTTCGTTGAAAATGAAT
TTAACTTGATTGATTTTACCACTAATGGTTTAAGAGTGGTCGATAAAGAAATTATCATTACAATGATTAAAGAAATTAAG
GAGAGTGATCGTGATAACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 14882.85 Da
• Isoelectric Point: 5.5500

>AT153995 WP_000124640.1 NZ_CP051316:c18670-18368 [Salmonella enterica subsp. enterica serovar Uganda]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 303 bp

>AT153995 NZ_CP067270:33372-33608 [Escherichia coli]
GTGATAACTGATAATACGCCGGAAAACATTAAAAAACTACGTGAAAAATTAGGGATTACGCAAACTGAATGCGGAAAACT
CTTTGGTGTAGGTTTAAGAACATGGCAAAAAAAAGAGGAAGCTAAAACCCATAACCGTTTGAGCTTATCAAAAGGGGAGT
TTGAATATCTTCTTTTAATGGCTGGGGAGCATCCTGATTTTGTTTTAGAAAAGAGAAAAAAAACTGAAAAAGAATAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9