Detailed information of TA system TA153966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 1859705..1860342
• Replicon: plasmid megaplasmid
• Accession: NZ_CP051295
• Organism: Ralstonia solanacearum strain CIAT_078

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F6G785
• Locus tag: G8D25_RS07540
• Protein ID: WP_014618424.1
• Coordinates: 1860013..1860342 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G8D25_RS07535
• Protein ID: WP_003262799.1
• Coordinates: 1859705..1860016 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G8D25_RS07495 (G8D25_07490)	1854901..1855554	-	654	WP_014618430.1	hypothetical protein	-
G8D25_RS07505 (G8D25_07500)	1855990..1856730	+	741	WP_003262792.1	metallophosphoesterase family protein	-
G8D25_RS07510 (G8D25_07505)	1856923..1857294	-	372	WP_014618429.1	hypothetical protein	-
G8D25_RS07515 (G8D25_07510)	1857575..1858408	+	834	WP_042548870.1	DUF2242 domain-containing protein	-
G8D25_RS07520 (G8D25_07515)	1858411..1858617	-	207	WP_014618427.1	hypothetical protein	-
G8D25_RS07525 (G8D25_07520)	1858990..1859301	+	312	WP_039551557.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
G8D25_RS07530 (G8D25_07525)	1859246..1859554	+	309	WP_003262797.1	type II toxin-antitoxin system YafQ family toxin	-
G8D25_RS07535 (G8D25_07530)	1859705..1860016	-	312	WP_003262799.1	transcriptional regulator	Antitoxin
G8D25_RS07540 (G8D25_07535)	1860013..1860342	-	330	WP_014618424.1	hypothetical protein	Toxin
G8D25_RS07545 (G8D25_07540)	1860393..1862225	-	1833	WP_247709151.1	propionate catabolism operon regulatory protein PrpR	-
G8D25_RS07550 (G8D25_07545)	1862407..1863308	+	902	Protein_1538	methylisocitrate lyase	-
G8D25_RS07555 (G8D25_07550)	1863397..1864506	+	1110	WP_168728883.1	2-methylcitrate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / flgG / fliM / fliI / acrB / tsr / adeG / cheY / cheB / cheW / motA / tsr / cyaB	1..1907373	1907373

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12413.89 Da
• Isoelectric Point: 5.2566

>T153966 WP_014618424.1 NZ_CP051295:c1860342-1860013 [Ralstonia solanacearum]
MDATPIELPGEHDLSDDEYSALQQELLAQPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGSSQFWMFVVYD
KDEAADLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 330 bp

>T153966 NZ_CP067261:782917-783327 [Klebsiella variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGTTGGTTCCCTGGCCGCTAAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTAGTGTTTGACTGCG
TACGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11620.59 Da
• Isoelectric Point: 10.3969

>AT153966 WP_003262799.1 NZ_CP051295:c1860016-1859705 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVEVRVLQPIDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT153966 NZ_CP067261:782670-782936 [Klebsiella variicola]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: F6G785