Detailed information of TA system TA153948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4612704..4613306
• Replicon: chromosome
• Accession: NZ_CP051263
• Organism: Escherichia coli CFT073

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HGG68_RS22185
• Protein ID: WP_000897302.1
• Coordinates: 4612704..4613015 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HGG68_RS22190
• Protein ID: WP_000356397.1
• Coordinates: 4613016..4613306 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGG68_RS22160	4608617..4609216	+	600	WP_001443191.1	glucose-1-phosphatase	-
HGG68_RS22165	4609210..4610082	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HGG68_RS22170	4610079..4610516	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HGG68_RS22175	4610561..4611502	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HGG68_RS22180	4611566..4612474	-	909	WP_001305059.1	alpha/beta hydrolase	-
HGG68_RS22185	4612704..4613015	+	312	WP_000897302.1	hypothetical protein	Toxin
HGG68_RS22190	4613016..4613306	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HGG68_RS22195	4613665..4613943	+	279	WP_001296612.1	hypothetical protein	-
HGG68_RS22200	4614339..4614557	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HGG68_RS22205	4614773..4615702	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HGG68_RS22210	4615699..4616334	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HGG68_RS22215	4616331..4617233	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T153948 WP_000897302.1 NZ_CP051263:4612704-4613015 [Escherichia coli CFT073]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T153948 NZ_CP067252:11941-12222 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT153948 WP_000356397.1 NZ_CP051263:4613016-4613306 [Escherichia coli CFT073]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT153948 NZ_CP067252:11700-11951 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: S1P416