Detailed information of TA system TA153946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3725143..3725942
• Replicon: chromosome
• Accession: NZ_CP051263
• Organism: Escherichia coli CFT073

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: HGG68_RS17885
• Protein ID: WP_000347252.1
• Coordinates: 3725478..3725942 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: HGG68_RS17880
• Protein ID: WP_001296435.1
• Coordinates: 3725143..3725478 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGG68_RS17865	3720928..3721698	-	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-
HGG68_RS17870	3721714..3723048	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
HGG68_RS17875	3723423..3724994	+	1572	WP_001273940.1	galactarate dehydratase	-
HGG68_RS17880	3725143..3725478	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HGG68_RS17885	3725478..3725942	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HGG68_RS17890	3725997..3726806	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HGG68_RS17895	3727055..3728335	+	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HGG68_RS17900	3728358..3728831	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HGG68_RS17905	3728842..3729621	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HGG68_RS17910	3729611..3730489	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HGG68_RS17915	3730507..3730941	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T153946 WP_000347252.1 NZ_CP051263:3725478-3725942 [Escherichia coli CFT073]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T153946 NZ_CP067251:c110163-109858 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT153946 WP_001296435.1 NZ_CP051263:3725143-3725478 [Escherichia coli CFT073]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT153946 NZ_CP067251:c110383-110165 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5