Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3132402..3133129 Replicon chromosome
Accession NZ_CP051263
Organism Escherichia coli CFT073

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag HGG68_RS15115 Protein ID WP_000547555.1
Coordinates 3132818..3133129 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag HGG68_RS15110 Protein ID WP_000126293.1
Coordinates 3132402..3132821 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGG68_RS15095 3128565..3130817 - 2253 WP_001107659.1 carbamoyltransferase HypF -
HGG68_RS15100 3130970..3131497 - 528 WP_001078777.1 electron transport protein HydN -
HGG68_RS15105 3131882..3132310 + 429 WP_000536064.1 hypothetical protein -
HGG68_RS15110 3132402..3132821 - 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
HGG68_RS15115 3132818..3133129 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
HGG68_RS15120 3133292..3134047 - 756 WP_001305268.1 hypothetical protein -
HGG68_RS15125 3134090..3134560 - 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
HGG68_RS15130 3134553..3134963 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
HGG68_RS15135 3134960..3135727 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
HGG68_RS15140 3135727..3136269 - 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
HGG68_RS15145 3136279..3137988 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T153943 WP_000547555.1 NZ_CP051263:c3133129-3132818 [Escherichia coli CFT073]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T153943 NZ_CP067250:3972219-3972683 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT153943 WP_000126293.1 NZ_CP051263:c3132821-3132402 [Escherichia coli CFT073]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT153943 NZ_CP067250:3971884-3972219 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References