Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1755788..1756350 Replicon chromosome
Accession NZ_CP051263
Organism Escherichia coli CFT073

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HGG68_RS08675 Protein ID WP_000605675.1
Coordinates 1756072..1756350 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HGG68_RS08670 Protein ID WP_000781370.1
Coordinates 1755788..1756072 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGG68_RS08655 1750972..1754019 + 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -
HGG68_RS08660 1754032..1754916 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
HGG68_RS08665 1754909..1755562 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
HGG68_RS08670 1755788..1756072 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HGG68_RS08675 1756072..1756350 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HGG68_RS08680 1756536..1757546 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
HGG68_RS08685 1757680..1759377 - 1698 WP_000433460.1 malate dehydrogenase -
HGG68_RS08690 1759534..1759671 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HGG68_RS08695 1759773..1759988 - 216 WP_000495766.1 biofilm-dependent modulation protein -
HGG68_RS08700 1760333..1760764 + 432 WP_000152307.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T153931 WP_000605675.1 NZ_CP051263:c1756350-1756072 [Escherichia coli CFT073]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T153931 NZ_CP067250:c1166754-1166536 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT153931 WP_000781370.1 NZ_CP051263:c1756072-1755788 [Escherichia coli CFT073]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT153931 NZ_CP067250:c1167154-1166780 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References