Detailed information of TA system TA153904

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 96513..96767
• Replicon: plasmid pSCZE2
• Accession: NZ_CP051224
• Organism: Escherichia coli strain SCZE5

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HF562_RS23960
• Protein ID: WP_001351576.1
• Coordinates: 96513..96719 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	96706..96767 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF562_RS23925	92065..92466	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HF562_RS23930	92399..92656	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HF562_RS23935	92749..93402	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HF562_RS23940	94341..95198	-	858	WP_168728579.1	incFII family plasmid replication initiator RepA	-
HF562_RS23945	95191..95673	-	483	WP_001273588.1	hypothetical protein	-
HF562_RS23950	95666..95740	-	75	WP_001442103.1	RepA leader peptide Tap	-
HF562_RS23955	95972..96229	-	258	WP_000083845.1	replication regulatory protein RepA	-
HF562_RS23960	96513..96719	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	96706..96767	+	62	NuclAT_1	-	Antitoxin
-	96706..96767	+	62	NuclAT_1	-	Antitoxin
-	96706..96767	+	62	NuclAT_1	-	Antitoxin
-	96706..96767	+	62	NuclAT_1	-	Antitoxin
HF562_RS23965	97023..97097	-	75	Protein_107	endonuclease	-
HF562_RS23970	97343..97555	-	213	WP_001299730.1	ANR family transcriptional regulator	-
HF562_RS23975	97691..98251	-	561	WP_000139315.1	fertility inhibition protein FinO	-
HF562_RS23980	98354..99214	-	861	WP_000704513.1	alpha/beta hydrolase	-
HF562_RS23985	99273..100019	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qacE / aadA2 / dfrA12 / oqxB / oqxA / mph(A) / aac(3)-IId / blaTEM-1A / floR / aph(3')-Ia / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..143259	143259

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T153904 WP_001351576.1 NZ_CP051224:c96719-96513 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T153904 NZ_CP067242:17695-18111 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT153904 NZ_CP051224:96706-96767 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin