Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4346358..4346855 Replicon chromosome
Accession NZ_CP051222
Organism Escherichia coli strain SCZE5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HF562_RS21110 Protein ID WP_001173343.1
Coordinates 4346358..4346531 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HF562_RS21115 Protein ID WP_000398619.1
Coordinates 4346559..4346855 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF562_RS21100 4343028..4344485 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
HF562_RS21105 4344722..4346239 + 1518 WP_001295090.1 lysine--tRNA ligase -
HF562_RS21110 4346358..4346531 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HF562_RS21115 4346559..4346855 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HF562_RS21120 4347082..4347354 - 273 WP_000405651.1 N-acetyltransferase -
HF562_RS21125 4347366..4347596 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
HF562_RS21130 4347777..4349408 + 1632 WP_001216477.1 sensor histidine kinase -
HF562_RS21135 4349405..4350124 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4337504..4346855 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T153903 WP_001173343.1 NZ_CP051222:c4346531-4346358 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T153903 NZ_CP067241:c4837743-4837369 [Escherichia coli]
ATGGCAAAAAGAACTGCGCTTTTTGATACCAATATCCTTATCGATTTGTTCAGCGGACGAATTGAGGCAAAACAGGCTCT
GGAGGCATGGCCCCTACAGAATGCGATTAGTCTGGTCACATGGATGGAGGTGCTGGTTGGCGCAAAAAAATATGATCAAG
AACATCGCACACGGATTGCAATGAGTGCGTTTAACGTTATTGGGATTTCGCAGGATATCGCGGAACGAAGCGTTGCTTTA
AGGCAGGAGTATAGAATGAAACTTCCAGATGCCATTATTCTGGCAACAGCACAGATTTATGGCTTTGAACTGGTAACGCG
AAATACGAGAGATTTTGCGGGAATAGCAGGTGTCGTCACACCCTATGAGCTGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT153903 WP_000398619.1 NZ_CP051222:c4346855-4346559 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT153903 NZ_CP067241:c4837985-4837743 [Escherichia coli]
ATGAGCGCTATGGCAGGTATGGATATGGGCAGAATTTTACTCGATTTATCCGATGAGGTGATTAAGCGACTTGATGATCT
CAAAGTGCAGCGCAATCTTCCTCGCGCAGAGCTATTACGAGAAGCTGTAGATCAATATCTGGAAAATCAATCGAAAACGA
CAATATCCAGTGCTTTTGGTATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References