Detailed information of TA system TA153878

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 106136..106390
• Replicon: plasmid pSCZP1
• Accession: NZ_CP051220
• Organism: Escherichia coli strain SFE8

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HF527_RS25065
• Protein ID: WP_181654380.1
• Coordinates: 106184..106390 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	106136..106197 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF527_RS25045	103352..104098	+	747	WP_021534916.1	conjugal transfer pilus acetylase TraX	-
HF527_RS25050	104153..104713	+	561	WP_106693617.1	fertility inhibition protein FinO	-
HF527_RS25055	104844..105056	+	213	WP_001311693.1	hypothetical protein	-
HF527_RS25490	105262..105348	+	87	Protein_119	endonuclease	-
HF527_RS25060	105529..105840	-	312	WP_000802277.1	hypothetical protein	-
-	106136..106197	-	62	NuclAT_1	-	Antitoxin
-	106136..106197	-	62	NuclAT_1	-	Antitoxin
-	106136..106197	-	62	NuclAT_1	-	Antitoxin
-	106136..106197	-	62	NuclAT_1	-	Antitoxin
HF527_RS25065	106184..106390	+	207	WP_181654380.1	type I toxin-antitoxin system Hok family toxin	Toxin
HF527_RS25070	106674..106931	+	258	WP_000083845.1	replication regulatory protein RepA	-
HF527_RS25075	107165..107230	+	66	Protein_123	RepA leader peptide Tap	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	afaC-I	1..107230	107230

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7820.35 Da
• Isoelectric Point: 8.8807

>T153878 WP_181654380.1 NZ_CP051220:106184-106390 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCVTVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T153878 NZ_CP067241:210977-211162 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT153878 NZ_CP051220:c106197-106136 [Escherichia coli]
TTAAGGTACTTCATACAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin