Detailed information of TA system TA153875

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69030..69299
• Replicon: plasmid pSCZP1
• Accession: NZ_CP051220
• Organism: Escherichia coli strain SFE8

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HF527_RS24855
• Protein ID: WP_001312861.1
• Coordinates: 69141..69299 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	69030..69095 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF527_RS25485	64568..64774	+	207	WP_000547971.1	hypothetical protein	-
HF527_RS24825	64800..65339	+	540	WP_000290813.1	single-stranded DNA-binding protein	-
HF527_RS24830	65402..65635	+	234	WP_000006030.1	DUF905 family protein	-
HF527_RS24835	65700..67658	+	1959	WP_168725381.1	ParB/RepB/Spo0J family partition protein	-
HF527_RS24840	67713..68147	+	435	WP_032217500.1	conjugation system SOS inhibitor PsiB	-
HF527_RS24845	68144..68904	+	761	Protein_77	plasmid SOS inhibition protein A	-
HF527_RS24850	68873..69061	-	189	WP_001299721.1	hypothetical protein	-
-	68873..69097	+	225	NuclAT_0	-	-
-	68873..69097	+	225	NuclAT_0	-	-
-	68873..69097	+	225	NuclAT_0	-	-
-	68873..69097	+	225	NuclAT_0	-	-
-	69030..69095	+	66	-	-	Antitoxin
HF527_RS24855	69141..69299	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HF527_RS24865	70221..70508	+	288	WP_000107535.1	hypothetical protein	-
HF527_RS24870	70627..71448	+	822	WP_001736720.1	DUF945 domain-containing protein	-
HF527_RS24875	71745..72347	-	603	WP_168725382.1	transglycosylase SLT domain-containing protein	-
HF527_RS24880	72669..73052	+	384	WP_096979339.1	relaxosome protein TraM	-
HF527_RS24885	73242..73928	+	687	WP_106693513.1	PAS domain-containing protein	-
HF527_RS24890	74022..74249	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	afaC-I	1..107230	107230

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T153875 WP_001312861.1 NZ_CP051220:69141-69299 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T153875 NZ_CP067237:c27800-27420 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT153875 NZ_CP051220:69030-69095 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin