Detailed information of TA system TA153871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4859177..4859779
• Replicon: chromosome
• Accession: NZ_CP051219
• Organism: Escherichia coli strain SFE8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HF527_RS23495
• Protein ID: WP_000897305.1
• Coordinates: 4859468..4859779 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HF527_RS23490
• Protein ID: WP_000356397.1
• Coordinates: 4859177..4859467 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF527_RS23465	4855103..4856005	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HF527_RS23470	4856002..4856637	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HF527_RS23475	4856634..4857563	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HF527_RS23480	4857893..4858135	-	243	WP_001086388.1	hypothetical protein	-
HF527_RS23485	4858354..4858572	-	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
HF527_RS23490	4859177..4859467	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HF527_RS23495	4859468..4859779	-	312	WP_000897305.1	hypothetical protein	Toxin
HF527_RS23500	4860008..4860916	+	909	WP_001162704.1	alpha/beta hydrolase	-
HF527_RS23505	4860980..4861921	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HF527_RS23510	4861966..4862403	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HF527_RS23515	4862400..4863272	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HF527_RS23520	4863266..4863865	-	600	WP_001295269.1	glucose-1-phosphatase	-
HF527_RS23525	4863964..4864749	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T153871 WP_000897305.1 NZ_CP051219:c4859779-4859468 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T153871 NZ_CP067235:c4864197-4863811 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT153871 WP_000356397.1 NZ_CP051219:c4859467-4859177 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT153871 NZ_CP067235:c4863821-4863492 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7