Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3904214..3904755 Replicon chromosome
Accession NZ_CP051219
Organism Escherichia coli strain SFE8

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HF527_RS19010 Protein ID WP_000615976.1
Coordinates 3904477..3904755 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HF527_RS19005 Protein ID WP_000729704.1
Coordinates 3904214..3904474 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF527_RS18980 3899997..3900782 - 786 WP_000207589.1 putative lateral flagellar export/assembly protein LafU -
HF527_RS18985 3900754..3902466 + 1713 Protein_3721 flagellar biosynthesis protein FlhA -
HF527_RS18990 3902571..3902849 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HF527_RS18995 3902842..3903198 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HF527_RS19000 3903255..3904028 - 774 WP_001396221.1 C40 family peptidase -
HF527_RS19005 3904214..3904474 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HF527_RS19010 3904477..3904755 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HF527_RS19015 3904911..3905651 + 741 WP_001225679.1 murein L,D-transpeptidase -
HF527_RS19020 3905622..3906389 - 768 WP_000333380.1 class II glutamine amidotransferase -
HF527_RS19025 3906595..3907173 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T153866 WP_000615976.1 NZ_CP051219:3904477-3904755 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T153866 NZ_CP067235:3630510-3630683 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGCGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT153866 WP_000729704.1 NZ_CP051219:3904214-3904474 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT153866 NZ_CP067235:3630186-3630482 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References