Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1950562..1951064 Replicon chromosome
Accession NZ_CP051219
Organism Escherichia coli strain SFE8

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HF527_RS09420 Protein ID WP_000767829.1
Coordinates 1950810..1951064 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HF527_RS09415 Protein ID WP_001259255.1
Coordinates 1950562..1950813 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF527_RS09390 1945739..1946806 - 1068 WP_000080095.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HF527_RS09395 1946806..1947876 - 1071 WP_000108976.1 histidinol-phosphate transaminase -
HF527_RS09400 1947873..1949177 - 1305 WP_001514311.1 histidinol dehydrogenase -
HF527_RS09405 1949183..1950082 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HF527_RS09410 1950228..1950278 - 51 WP_001364200.1 his operon leader peptide -
HF527_RS09415 1950562..1950813 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HF527_RS09420 1950810..1951064 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HF527_RS09425 1951147..1951971 + 825 WP_000754737.1 SDR family oxidoreductase -
HF527_RS09430 1952017..1952946 + 930 WP_000803341.1 LysR family transcriptional regulator -
HF527_RS09435 1953161..1953223 + 63 WP_010723108.1 membrane protein YoeI -
HF527_RS09440 1953213..1954571 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HF527_RS09445 1954750..1955808 + 1059 WP_000492340.1 transport protein YeeE -
HF527_RS09450 1955822..1956049 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T153853 WP_000767829.1 NZ_CP051219:1950810-1951064 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T153853 NZ_CP067234:c63568-63188 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACCTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT153853 WP_001259255.1 NZ_CP051219:1950562-1950813 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT153853 NZ_CP067234:c63789-63568 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References