Detailed information of TA system TA153846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4782963..4783717
• Replicon: chromosome
• Accession: NZ_CP051218
• Organism: Salmonella sp. SCFS4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4Y6MJG6
• Locus tag: HF553_RS23005
• Protein ID: WP_000558165.1
• Coordinates: 4782963..4783274 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HF553_RS23010
• Protein ID: WP_001259009.1
• Coordinates: 4783271..4783717 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF553_RS22975	4778629..4779531	+	903	WP_051129234.1	formate dehydrogenase subunit beta	-
HF553_RS22980	4779528..4780163	+	636	WP_000829028.1	formate dehydrogenase cytochrome b556 subunit	-
HF553_RS22985	4780160..4781089	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
HF553_RS22990	4781127..4781501	-	375	WP_000238494.1	type II toxin-antitoxin system VapC family toxin	-
HF553_RS22995	4781501..4781743	-	243	WP_001523745.1	ribbon-helix-helix domain-containing protein	-
HF553_RS23000	4781948..4782877	+	930	WP_001162861.1	alpha/beta hydrolase	-
HF553_RS23005	4782963..4783274	+	312	WP_000558165.1	type II toxin-antitoxin system HigB family toxin	Toxin
HF553_RS23010	4783271..4783717	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
HF553_RS23015	4783732..4784673	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
HF553_RS23020	4784718..4785155	-	438	WP_000560969.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HF553_RS23025	4785152..4786024	-	873	WP_000921423.1	virulence factor BrkB family protein	-
HF553_RS23030	4786018..4786617	-	600	WP_023227076.1	glucose-1-phosphatase	-
HF553_RS23035	4786808..4787611	-	804	WP_000059689.1	DeoR/GlpR family DNA-binding transcription regulator	-
HF553_RS23040	4787645..4788541	-	897	WP_023227075.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12433.34 Da
• Isoelectric Point: 8.4020

>T153846 WP_000558165.1 NZ_CP051218:4782963-4783274 [Salmonella sp. SCFS4]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNEE

Nucleotide

• Length: 312 bp

>T153846 NZ_CP067232:3655746-3655849 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT153846 WP_001259009.1 NZ_CP051218:4783271-4783717 [Salmonella sp. SCFS4]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT153846 NZ_CP067232:c3655887-3655616 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ATGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A4Y6MJG6