Detailed information of TA system TA153725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2535686..2536299
• Replicon: chromosome
• Accession: NZ_CP051183
• Organism: Bermanella marisrubri strain RED65

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q1N2S2
• Locus tag: HF888_RS11730
• Protein ID: WP_007016198.1
• Coordinates: 2535686..2535976 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HF888_RS11735
• Protein ID: WP_007016197.1
• Coordinates: 2535985..2536299 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF888_RS11720	2531834..2534728	+	2895	WP_007016200.1	aminomethyl-transferring glycine dehydrogenase	-
HF888_RS11725	2534948..2535517	+	570	WP_007016199.1	hypothetical protein	-
HF888_RS11730	2535686..2535976	+	291	WP_007016198.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HF888_RS11735	2535985..2536299	+	315	WP_007016197.1	HigA family addiction module antidote protein	Antitoxin
HF888_RS11740	2536354..2536683	+	330	WP_007016196.1	hypothetical protein	-
HF888_RS11745	2536680..2537603	-	924	WP_007016195.1	LysR family transcriptional regulator	-
HF888_RS11750	2537753..2538379	+	627	WP_007016194.1	glutathione S-transferase family protein	-
HF888_RS11755	2538456..2539526	-	1071	WP_007016193.1	alkene reductase	-
HF888_RS11760	2539892..2540968	+	1077	WP_007016192.1	ribosome small subunit-dependent GTPase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (11-97)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11331.05 Da
• Isoelectric Point: 9.6573

>T153725 WP_007016198.1 NZ_CP051183:2535686-2535976 [Bermanella marisrubri]
MIESFKEDWLSNYYWEAVKSKKIPSDIESALNRKLSIIHAAVEEKDLRCPPGNRFEHLQGNLKGWCSIRVSKQYRLIFKW
ADGKATQLYLDAHKYK

Nucleotide

• Length: 291 bp

>T153725 NZ_CP067060:797904-798314 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11543.51 Da
• Isoelectric Point: 8.0462

>AT153725 WP_007016197.1 NZ_CP051183:2535985-2536299 [Bermanella marisrubri]
MTTEFRIPGHQPTSVGIMLKEEFLDPMKISQGELAKAMGVSRKTVNELCGNKRGITAETALLLSKVLGTTPEFWINLQIM
NDLWATSHSEKMKDKLEKATPLVA

Nucleotide

• Length: 315 bp

>AT153725 NZ_CP067060:797657-797923 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: Q1N2S2