Detailed information of TA system TA153697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1181290..1182010
• Replicon: chromosome
• Accession: NZ_CP051168
• Organism: [Phormidium] sp. ETS-05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0M1JU66
• Locus tag: HEQ85_RS05250
• Protein ID: WP_054466246.1
• Coordinates: 1181290..1181577 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HEQ85_RS05255
• Protein ID: WP_199248607.1
• Coordinates: 1181699..1182010 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HEQ85_RS05225	1176650..1176838	+	189	WP_199248603.1	hypothetical protein	-
HEQ85_RS05230	1176898..1177167	+	270	WP_199248604.1	hypothetical protein	-
HEQ85_RS05235	1177170..1177496	+	327	WP_199248605.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HEQ85_RS05240	1178028..1179184	-	1157	Protein_1027	transposase	-
HEQ85_RS05245	1179750..1180994	+	1245	WP_199248606.1	transposase	-
HEQ85_RS05250	1181290..1181577	+	288	WP_054466246.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HEQ85_RS05255	1181699..1182010	+	312	WP_199248607.1	HigA family addiction module antidote protein	Antitoxin
HEQ85_RS05260	1182169..1182342	+	174	WP_199248608.1	hypothetical protein	-
HEQ85_RS05265	1182363..1182605	-	243	WP_199248609.1	type II toxin-antitoxin system VapB family antitoxin	-
HEQ85_RS05270	1182710..1183237	+	528	Protein_1033	DUF29 domain-containing protein	-
HEQ85_RS05275	1183745..1184044	-	300	WP_199248610.1	hypothetical protein	-
HEQ85_RS05280	1184229..1184366	-	138	WP_199248611.1	hypothetical protein	-
HEQ85_RS05285	1184491..1184745	+	255	WP_199248612.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
HEQ85_RS05290	1184742..1184945	+	204	WP_199248613.1	Txe/YoeB family addiction module toxin	-
HEQ85_RS05295	1185553..1186005	-	453	WP_199248614.1	DUF29 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1144979..1182010	37031

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-81)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-71)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11013.64 Da
• Isoelectric Point: 10.2453

>T153697 WP_054466246.1 NZ_CP051168:1181290-1181577 [[Phormidium] sp. ETS-05]
MIKDFKNKRLAAFFREGNNKGIPKEFEKRIRVRLEALDSASCLDDLKIPGYNLHELKGDRKETWSIALSGNWRITFKFEQ
GKGEAFGQNTFLFVS

Nucleotide

• Length: 288 bp

>T153697 NZ_CP067043:3863750-3864496 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATACGTGTACGCATCCTGGCGGTATGA
AGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTATTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGAGTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGGACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11541.25 Da
• Isoelectric Point: 4.6533

>AT153697 WP_199248607.1 NZ_CP051168:1181699-1182010 [[Phormidium] sp. ETS-05]
MQNLENIVNNRLKRPAHPGEVLADILDDLQITEAEFAKALNVSAETVNEIIDGKKAITIDLAIRLGKALGNGPQIWLNLQ
QKIDIWDAMTVDREQYEKVHAIV

Nucleotide

• Length: 312 bp

>AT153697 NZ_CP067043:3864497-3864667 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0M1JU66