Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2163691..2164418 Replicon chromosome
Accession NZ_CP051158
Organism Escherichia coli strain 17MR471

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HF575_RS10225 Protein ID WP_000550189.1
Coordinates 2163691..2164005 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HF575_RS10230 Protein ID WP_000560266.1
Coordinates 2164002..2164418 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF575_RS10205 2159849..2160835 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
HF575_RS10210 2160914..2161606 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
HF575_RS10215 2161683..2162135 - 453 WP_001336107.1 M48 family metallopeptidase -
HF575_RS10220 2162271..2163407 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HF575_RS10225 2163691..2164005 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HF575_RS10230 2164002..2164418 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HF575_RS10235 2164463..2166481 - 2019 WP_176607764.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HF575_RS10240 2166907..2169258 - 2352 WP_061093122.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T153595 WP_000550189.1 NZ_CP051158:2163691-2164005 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T153595 NZ_CP066983:353467-353601 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT153595 WP_000560266.1 NZ_CP051158:2164002-2164418 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT153595 NZ_CP066983:353235-353387 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References