Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2119273..2120072 Replicon chromosome
Accession NZ_CP051158
Organism Escherichia coli strain 17MR471

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag HF575_RS10000 Protein ID WP_000347273.1
Coordinates 2119273..2119737 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HF575_RS10005 Protein ID WP_001307405.1
Coordinates 2119737..2120072 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF575_RS09970 2114274..2114708 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
HF575_RS09975 2114726..2115604 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HF575_RS09980 2115594..2116373 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HF575_RS09985 2116384..2116857 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
HF575_RS09990 2116880..2118160 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
HF575_RS09995 2118409..2119218 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HF575_RS10000 2119273..2119737 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HF575_RS10005 2119737..2120072 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HF575_RS10010 2120179..2121150 - 972 WP_170386723.1 IS30-like element ISApl1 family transposase -
HF575_RS10015 2121293..2122864 - 1572 WP_001273753.1 galactarate dehydratase -
HF575_RS10020 2123239..2124573 + 1335 WP_061093123.1 galactarate/glucarate/glycerate transporter GarP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2120179..2121102 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T153594 WP_000347273.1 NZ_CP051158:c2119737-2119273 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T153594 NZ_CP066983:353235-353387 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT153594 WP_001307405.1 NZ_CP051158:c2120072-2119737 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT153594 NZ_CP066983:353467-353601 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References