Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1034734..1035231 Replicon chromosome
Accession NZ_CP051158
Organism Escherichia coli strain 17MR471

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HF575_RS04895 Protein ID WP_001173343.1
Coordinates 1034734..1034907 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HF575_RS04900 Protein ID WP_000398619.1
Coordinates 1034935..1035231 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF575_RS04885 1031404..1032861 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HF575_RS04890 1033098..1034615 + 1518 WP_001295090.1 lysine--tRNA ligase -
HF575_RS04895 1034734..1034907 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HF575_RS04900 1034935..1035231 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HF575_RS04905 1035458..1035730 - 273 WP_000405651.1 N-acetyltransferase -
HF575_RS04910 1035742..1035972 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
HF575_RS04915 1036153..1037784 + 1632 WP_001216477.1 sensor histidine kinase -
HF575_RS04920 1037781..1038500 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1025880..1035231 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T153593 WP_001173343.1 NZ_CP051158:c1034907-1034734 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T153593 NZ_CP066982:3613753-3614244 [Xanthomonas campestris pv. campestris]
ATGACGGTATCGGCGCCGGAACCGCTGACAGCGCATCACCGGGTCGAACCGTTCGCATCGGGCGTGGAGAGCCTGGATCA
CTGGCTCAAGCGCCGCGCCCTGAAAAATCAGGCAACCGGTGCGTCACGGACCTTCGTTGCCTGCGAGGATGACCGCGTGG
TGGCCTATTACGCCCTGGCGTCGAGTGCGGTCGCCGTGGACGCGACGCCCGGCCGCTTCCGTCGCAACATGCCGGACCCG
ATCCCCGTGGTGGTGCTTGGGAGGCTTGCTGTTGACCAATCCCTGCATGGCAGAGGTTTTGGCCGCGCCCTGATGCAGGA
CGCTGGCAAGCGCATCCTGCATGCGGCCGACACCATCGGCATTCGCGGGCTGCTCGTGCATGCGCTCTCGGCGGACGCTA
AGGCGTTTTACGAGCGCATCGGCTTCGAACCTTCCCCGCTGGATCCCATGGTGCTGGTCGTCACGCTGGAAGACCTGAAA
GCCAGTCTGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT153593 WP_000398619.1 NZ_CP051158:c1035231-1034935 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT153593 NZ_CP066982:3613466-3613756 [Xanthomonas campestris pv. campestris]
ATGTCCACACCCAACACTACGCCCGGCAAGCGCGAAACCTTGAATCTCCGCATCAAGCCGGAGGAGCGCAGCCTGATCGA
CCGGGCCGCCAAGGCGCGCGGGAAGAACCGCACAGATTTCATGCTGGACGCAGCGCGCTCGGCGGCCGAGGAAGCCTTGC
TGGACCAGACCCTTATCGCGGCCAGCCCGGACGCCTACGCCGCCTTTTTGGCACGGCTGGACATGCCGCCGCAGCCGAAC
GCGCGCCTGCGCAAGACGATGCAGACGCCCGCTCCTTGGGAGAAGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References