Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 511551..512245 Replicon chromosome
Accession NZ_CP051158
Organism Escherichia coli strain 17MR471

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HF575_RS02425 Protein ID WP_001263489.1
Coordinates 511551..511949 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HF575_RS02430 Protein ID WP_000554758.1
Coordinates 511952..512245 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 507139..507219 - 81 NuclAT_9 - -
- 507139..507219 - 81 NuclAT_9 - -
- 507139..507219 - 81 NuclAT_9 - -
- 507139..507219 - 81 NuclAT_9 - -
HF575_RS02400 507815..508273 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
HF575_RS02405 508534..509991 + 1458 WP_032266102.1 cytosol nonspecific dipeptidase -
HF575_RS02410 510048..510605 - 558 WP_001352051.1 peptide chain release factor H -
HF575_RS02415 510517..510783 - 267 WP_001295202.1 hypothetical protein -
HF575_RS02420 511089..511541 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
HF575_RS02425 511551..511949 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HF575_RS02430 511952..512245 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HF575_RS02435 512297..513352 - 1056 WP_001226164.1 DNA polymerase IV -
HF575_RS02440 513423..514208 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HF575_RS02445 514153..515892 + 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
HF575_RS02450 516116..516613 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / clpV/tssH / hcp1/tssD1 511551..538265 26714


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T153589 WP_001263489.1 NZ_CP051158:c511949-511551 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T153589 NZ_CP066981:c4578043-4577909 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT153589 WP_000554758.1 NZ_CP051158:c512245-511952 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT153589 NZ_CP066981:c4578275-4578123 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References