Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3780669..3781171 Replicon chromosome
Accession NZ_CP051001
Organism Escherichia coli O157:H16 str. 98-3133

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BY40_RS18780 Protein ID WP_000767829.1
Coordinates 3780669..3780923 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BY40_RS18785 Protein ID WP_001259255.1
Coordinates 3780920..3781171 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BY40_RS18760 (3777162) 3777162..3778520 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
BY40_RS18765 (3778510) 3778510..3778572 - 63 WP_010723108.1 membrane protein YoeI -
BY40_RS18770 (3778787) 3778787..3779716 - 930 WP_000803352.1 LysR substrate-binding domain-containing protein -
BY40_RS18775 (3779762) 3779762..3780586 - 825 WP_000754746.1 SDR family oxidoreductase -
BY40_RS18780 (3780669) 3780669..3780923 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BY40_RS18785 (3780920) 3780920..3781171 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BY40_RS18790 (3781454) 3781454..3781504 + 51 WP_001364200.1 his operon leader peptide -
BY40_RS18795 (3781650) 3781650..3782549 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
BY40_RS18800 (3782555) 3782555..3783859 + 1305 WP_001373880.1 histidinol dehydrogenase -
BY40_RS18805 (3783856) 3783856..3784926 + 1071 WP_000108988.1 histidinol-phosphate transaminase -
BY40_RS18810 (3784926) 3784926..3785993 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T153524 WP_000767829.1 NZ_CP051001:c3780923-3780669 [Escherichia coli O157:H16 str. 98-3133]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T153524 NZ_CP066945:c4584000-4583866 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT153524 WP_001259255.1 NZ_CP051001:c3781171-3780920 [Escherichia coli O157:H16 str. 98-3133]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT153524 NZ_CP066945:c4584229-4584077 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References