Detailed information of TA system TA153509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1859580..1860274
• Replicon: chromosome
• Accession: NZ_CP051001
• Organism: Escherichia coli O157:H16 str. 98-3133

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: BY40_RS09045
• Protein ID: WP_001263489.1
• Coordinates: 1859876..1860274 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: BY40_RS09040
• Protein ID: WP_000554758.1
• Coordinates: 1859580..1859873 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BY40_RS09020 (1855212)	1855212..1855709	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
BY40_RS09025 (1855933)	1855933..1857645	-	1713	Protein_1761	flagellar biosynthesis protein FlhA	-
BY40_RS09030 (1857617)	1857617..1858402	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
BY40_RS09035 (1858473)	1858473..1859528	+	1056	WP_001226164.1	DNA polymerase IV	-
BY40_RS09040 (1859580)	1859580..1859873	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BY40_RS09045 (1859876)	1859876..1860274	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BY40_RS09050 (1860284)	1860284..1860655	+	372	Protein_1766	GNAT family N-acetyltransferase	-
BY40_RS09055 (1860686)	1860686..1861654	+	969	WP_073970534.1	IS5 family transposase	-
BY40_RS09060 (1861710)	1861710..1861802	+	93	Protein_1768	GNAT family N-acetyltransferase	-
BY40_RS09065 (1862120)	1862120..1862326	+	207	Protein_1769	RtcB family protein	-
BY40_RS09070 (1862322)	1862322..1862843	+	522	Protein_1770	peptide chain release factor H	-
BY40_RS09075 (1862900)	1862900..1864357	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
BY40_RS09080 (1864618)	1864618..1865076	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	hcp1/tssD1 / tssA / rhs/PAAR / gmhA/lpcA	1830146..1860664	30518
	-	flank	IS/Tn	-	-	1860731..1861654	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T153509 WP_001263489.1 NZ_CP051001:1859876-1860274 [Escherichia coli O157:H16 str. 98-3133]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T153509 NZ_CP066934:5036044-5036199 [Xanthomonas campestris pv. campestris]
GTGACCCTGCTCGATACCAATGTCATCTTCGGGCCCAATCTGCATGTCGTCGCCTGGATCGATGTGCAGGCCGTTGAAAC
ACTGTTCCTGTCGGTCGTGACGGTGGCCGAATTGCGATCTGGAGACATTCAGGATTTCAGACCCCTGATGAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT153509 WP_000554758.1 NZ_CP051001:1859580-1859873 [Escherichia coli O157:H16 str. 98-3133]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT153509 NZ_CP066934:5035793-5036047 [Xanthomonas campestris pv. campestris]
ATGCCTTCTTTCACCGTCCGCAACATCCCGGAGGATGTGCATCGCGCCATCTGGGCACGTGCTGCCCAACACGGGCGCAG
CACCGAGGCGGAGATCCGCGCGATCCTTGAATCAGCAGCCAAGCCAGCCGACCAGGTCAAACTTGGCGCGTTGCTGGTGG
ACATCGGCCGCGACGCTGGCCTGACAGCAAAAGAGGCCGACGCCTTCGCCAAGTTACGCGACAAGGCCGCGATCAAGCCG
ATCGGTTTGAAGTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3