Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1853536..1854077 Replicon chromosome
Accession NZ_CP051001
Organism Escherichia coli O157:H16 str. 98-3133

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag BY40_RS09005 Protein ID WP_000615983.1
Coordinates 1853536..1853814 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag BY40_RS09010 Protein ID WP_000729703.1
Coordinates 1853817..1854077 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BY40_RS08990 (1851118) 1851118..1851696 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
BY40_RS08995 (1851902) 1851902..1852669 + 768 WP_000333380.1 class II glutamine amidotransferase -
BY40_RS09000 (1852640) 1852640..1853380 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
BY40_RS09005 (1853536) 1853536..1853814 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
BY40_RS09010 (1853817) 1853817..1854077 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
BY40_RS09015 (1854287) 1854287..1855036 + 750 WP_000056849.1 C40 family peptidase -
BY40_RS09020 (1855212) 1855212..1855709 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
BY40_RS09025 (1855933) 1855933..1857645 - 1713 Protein_1761 flagellar biosynthesis protein FlhA -
BY40_RS09030 (1857617) 1857617..1858402 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - hcp1/tssD1 / tssA / rhs/PAAR / gmhA/lpcA 1830146..1860664 30518


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T153508 WP_000615983.1 NZ_CP051001:c1853814-1853536 [Escherichia coli O157:H16 str. 98-3133]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T153508 NZ_CP066934:c4704819-4704667 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT153508 WP_000729703.1 NZ_CP051001:c1854077-1853817 [Escherichia coli O157:H16 str. 98-3133]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT153508 NZ_CP066934:c4704590-4704456 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References