Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 149747..150546 Replicon chromosome
Accession NZ_CP051001
Organism Escherichia coli O157:H16 str. 98-3133

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag BY40_RS00750 Protein ID WP_000347273.1
Coordinates 150082..150546 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag BY40_RS00745 Protein ID WP_001307405.1
Coordinates 149747..150082 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BY40_RS00730 (145532) 145532..146302 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
BY40_RS00735 (146318) 146318..147652 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
BY40_RS00740 (148027) 148027..149598 + 1572 WP_001273753.1 galactarate dehydratase -
BY40_RS00745 (149747) 149747..150082 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
BY40_RS00750 (150082) 150082..150546 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
BY40_RS00755 (150601) 150601..151410 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
BY40_RS00760 (151659) 151659..152939 + 1281 Protein_149 tagatose-bisphosphate aldolase subunit KbaZ -
BY40_RS00765 (152962) 152962..153435 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
BY40_RS00770 (153446) 153446..154225 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
BY40_RS00775 (154215) 154215..155093 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
BY40_RS00780 (155111) 155111..155545 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 140599..165058 24459


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T153505 WP_000347273.1 NZ_CP051001:150082-150546 [Escherichia coli O157:H16 str. 98-3133]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T153505 NZ_CP066933:c4682810-4682658 [Xanthomonas campestris pv. campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT153505 WP_001307405.1 NZ_CP051001:149747-150082 [Escherichia coli O157:H16 str. 98-3133]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT153505 NZ_CP066933:c4682581-4682447 [Xanthomonas campestris pv. campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References