Detailed information of TA system TA153500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 55103..55814
• Replicon: plasmid pF8704-2_2
• Accession: NZ_CP050999
• Organism: Escherichia coli O39:NM str. F8704-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: P411_RS27495
• Protein ID: WP_000162415.1
• Coordinates: 55512..55814 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: P411_RS27490
• Protein ID: WP_000806445.1
• Coordinates: 55103..55441 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P411_RS27460	50398..51219	-	822	WP_259407116.1	hypothetical protein	-
P411_RS27465	51565..52218	+	654	WP_000410952.1	hypothetical protein	-
P411_RS27470	52547..52876	+	330	WP_000542383.1	hypothetical protein	-
P411_RS27475	52869..54062	+	1194	WP_001112633.1	hypothetical protein	-
P411_RS27480	54096..54824	-	729	WP_000986754.1	hypothetical protein	-
P411_RS27485	54861..55046	-	186	Protein_76	hypothetical protein	-
P411_RS27490	55103..55441	-	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
P411_RS27495	55512..55814	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
P411_RS27500	55977..56768	+	792	WP_000532305.1	hypothetical protein	-
P411_RS27505	56765..57532	+	768	WP_000203290.1	hypothetical protein	-
P411_RS27510	57536..58516	+	981	WP_000046499.1	hypothetical protein	-
P411_RS27515	58513..59166	+	654	WP_000828873.1	hypothetical protein	-
P411_RS27520	59226..60131	+	906	WP_001258014.1	recombination-associated protein RdgC	-
P411_RS27525	60145..60794	+	650	Protein_84	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..74525	74525

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T153500 WP_000162415.1 NZ_CP050999:c55814-55512 [Escherichia coli O39:NM str. F8704-2]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T153500 NZ_CP066931:c4774247-4774095 [Xanthomonas campestris pv. raphani]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT153500 WP_000806445.1 NZ_CP050999:c55441-55103 [Escherichia coli O39:NM str. F8704-2]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT153500 NZ_CP066931:c4774018-4773884 [Xanthomonas campestris pv. raphani]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3