Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 2782179..2782781 Replicon chromosome
Accession NZ_CP050998
Organism Escherichia coli O39:NM str. F8704-2

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag P411_RS14190 Protein ID WP_000897305.1
Coordinates 2782470..2782781 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag P411_RS14185 Protein ID WP_000356397.1
Coordinates 2782179..2782469 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P411_RS14160 (2777744) 2777744..2778379 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
P411_RS14165 (2778376) 2778376..2779305 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
P411_RS14170 (2779704) 2779704..2780684 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
P411_RS14175 (2780914) 2780914..2781156 - 243 WP_001086388.1 protein YiiF -
P411_RS14180 (2781375) 2781375..2781593 - 219 WP_001295676.1 CopG family transcriptional regulator -
P411_RS14185 (2782179) 2782179..2782469 - 291 WP_000356397.1 NadS family protein Antitoxin
P411_RS14190 (2782470) 2782470..2782781 - 312 WP_000897305.1 hypothetical protein Toxin
P411_RS14195 (2783010) 2783010..2783918 + 909 WP_001299484.1 alpha/beta hydrolase -
P411_RS14200 (2783982) 2783982..2784923 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
P411_RS14205 (2784968) 2784968..2785405 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
P411_RS14210 (2785402) 2785402..2786274 - 873 WP_000920762.1 virulence factor BrkB family protein -
P411_RS14215 (2786268) 2786268..2786867 - 600 WP_001295269.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2779704..2780684 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T153483 WP_000897305.1 NZ_CP050998:c2782781-2782470 [Escherichia coli O39:NM str. F8704-2]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T153483 NZ_CP066925:355411-355563 [Xanthomonas campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCTGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT153483 WP_000356397.1 NZ_CP050998:c2782469-2782179 [Escherichia coli O39:NM str. F8704-2]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT153483 NZ_CP066925:355640-355774 [Xanthomonas campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References