Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1560961..1561502 Replicon chromosome
Accession NZ_CP050998
Organism Escherichia coli O39:NM str. F8704-2

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag P411_RS08385 Protein ID WP_000615976.1
Coordinates 1560961..1561239 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag P411_RS08390 Protein ID WP_000729704.1
Coordinates 1561242..1561502 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P411_RS08370 (1558543) 1558543..1559121 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
P411_RS08375 (1559327) 1559327..1560094 + 768 WP_000333380.1 class II glutamine amidotransferase -
P411_RS08380 (1560065) 1560065..1560805 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
P411_RS08385 (1560961) 1560961..1561239 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
P411_RS08390 (1561242) 1561242..1561502 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
P411_RS08395 (1561688) 1561688..1562461 + 774 WP_000543899.1 C40 family peptidase -
P411_RS08400 (1562518) 1562518..1562874 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
P411_RS08405 (1562867) 1562867..1563145 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
P411_RS08410 (1563250) 1563250..1564962 - 1713 Protein_1577 flagellar biosynthesis protein FlhA -
P411_RS08415 (1564934) 1564934..1565719 + 786 WP_000207555.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T153477 WP_000615976.1 NZ_CP050998:c1561239-1560961 [Escherichia coli O39:NM str. F8704-2]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T153477 NZ_CP066922:394779-394931 [Xanthomonas campestris]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTCTCGGCCGGCATGATGACTGGCTGTAACACCATGGCTGGTGC
CGGAAAGGACATGCAGGGTGCGGGTGACAAGGTCGAGAAGACCGCCGACAAATGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT153477 WP_000729704.1 NZ_CP050998:c1561502-1561242 [Escherichia coli O39:NM str. F8704-2]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT153477 NZ_CP066922:395011-395145 [Xanthomonas campestris]
ATGAAGCGTGCAATTGCATTGTTGGTGCTGTCGATGTTGTCGGTTGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCCGCGCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References