Detailed information of TA system TA153463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1347700..1348286
• Replicon: chromosome
• Accession: NZ_CP050951
• Organism: Pseudomonas putida strain JQ581

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: A3L25_RS06105
• Protein ID: WP_081238120.1
• Coordinates: 1348008..1348286 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: A3L25_RS06100
• Protein ID: WP_003252274.1
• Coordinates: 1347700..1347999 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3L25_RS06075	1343502..1344311	+	810	WP_169725100.1	tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA	-
A3L25_RS06080	1344472..1344882	-	411	WP_059394610.1	SufE family protein	-
A3L25_RS06085	1344879..1346084	-	1206	WP_063426571.1	cysteine desulfurase	-
A3L25_RS06090	1346162..1347196	-	1035	WP_016485200.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
A3L25_RS06095	1347228..1347575	-	348	WP_003252272.1	ArsC family reductase	-
A3L25_RS06100	1347700..1347999	-	300	WP_003252274.1	type II toxin-antitoxin system antitoxin GraA	Antitoxin
A3L25_RS06105	1348008..1348286	-	279	WP_081238120.1	type II toxin-antitoxin system toxin GraT	Toxin
A3L25_RS06110	1348539..1350185	+	1647	WP_063426575.1	Na+/H+ antiporter	-
A3L25_RS06115	1350327..1351523	-	1197	WP_063426577.1	succinyldiaminopimelate transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-90)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10487.86 Da
• Isoelectric Point: 7.8928

>T153463 WP_081238120.1 NZ_CP050951:c1348286-1348008 [Pseudomonas putida]
MIQSFSCADTEALFTTGKTRRWSVIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRAGQHSIRVNDQWRLCFTWTE
HGPINVEIVDYH

Nucleotide

• Length: 279 bp

>T153463 NZ_CP066915:c3476482-3476380 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10891.52 Da
• Isoelectric Point: 5.6714

>AT153463 WP_003252274.1 NZ_CP050951:c1347999-1347700 [Pseudomonas putida]
MLKNGMRPIHPGEILREDFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Nucleotide

• Length: 300 bp

>AT153463 NZ_CP066915:3476373-3476518 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT