Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 188875..189411 Replicon plasmid Ps2192A-p7
Accession NZ_CP050945
Organism Piscirickettsia salmonis strain Ps-2192A

Toxin (Protein)


Gene name yoeB Uniprot ID A0A6I5YBD1
Locus tag GW536_RS19365 Protein ID WP_016211910.1
Coordinates 189139..189411 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A0B8U9U2
Locus tag GW536_RS19360 Protein ID WP_032126795.1
Coordinates 188875..189135 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GW536_RS19325 183939..184265 - 327 WP_016212298.1 hypothetical protein -
GW536_RS19330 184506..184982 + 477 WP_051307374.1 Clp protease ClpP -
GW536_RS19335 185097..186217 + 1121 Protein_233 IS3 family transposase -
GW536_RS19340 186221..186727 - 507 WP_129556449.1 IS630 family transposase -
GW536_RS19345 186742..187107 - 366 WP_054300249.1 IS630 transposase-related protein -
GW536_RS19350 187221..187922 - 702 WP_129556698.1 IS6 family transposase -
GW536_RS19355 188012..188602 - 591 WP_016211912.1 site-specific integrase -
GW536_RS19360 188875..189135 + 261 WP_032126795.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GW536_RS19365 189139..189411 + 273 WP_016211910.1 Txe/YoeB family addiction module toxin Toxin
GW536_RS19370 189737..190858 - 1122 WP_016211913.1 hypothetical protein -
GW536_RS19375 191291..191689 + 399 WP_075273786.1 helix-turn-helix domain-containing protein -
GW536_RS19380 191698..192853 - 1156 Protein_242 IS3 family transposase -
GW536_RS19385 192913..193515 + 603 Protein_243 IS30 family transposase -
GW536_RS19390 193852..194226 + 375 WP_016212499.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..212888 212888
- flank IS/Tn - - 187221..187922 701


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-74)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10739.22 Da        Isoelectric Point: 7.8926

>T153461 WP_016211910.1 NZ_CP050945:189139-189411 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRTPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
IAACRYHYDQ

Download         Length: 273 bp

>T153461 NZ_CP066915:c1330383-1330165 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9555.93 Da        Isoelectric Point: 5.1167

>AT153461 WP_032126795.1 NZ_CP050945:188875-189135 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Download         Length: 261 bp

>AT153461 NZ_CP066915:c1330784-1330410 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I5YBD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0B8U9U2

References