Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 609172..609778 Replicon chromosome
Accession NZ_CP050869
Organism Saccharolobus solfataricus strain POZ149

Toxin (Protein)


Gene name vapC Uniprot ID Q97YS3
Locus tag HFC64_RS03065 Protein ID WP_009993017.1
Coordinates 609172..609564 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A3G8ES43
Locus tag HFC64_RS03070 Protein ID WP_009993015.1
Coordinates 609554..609778 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HFC64_RS03040 (HFC64_03015) 605193..605540 + 348 WP_014511635.1 HEPN domain-containing protein -
HFC64_RS03045 (HFC64_03020) 605531..605872 + 342 WP_009990179.1 nucleotidyltransferase domain-containing protein -
HFC64_RS03050 (HFC64_03025) 606306..606893 + 588 Protein_617 ISNCY family transposase -
HFC64_RS03055 (HFC64_03030) 607081..608133 - 1053 WP_063492773.1 ISH3 family transposase -
HFC64_RS03060 (HFC64_03035) 608251..608729 + 479 Protein_619 ISNCY family transposase -
HFC64_RS03065 (HFC64_03040) 609172..609564 - 393 WP_009993017.1 type II toxin-antitoxin system VapC family toxin Toxin
HFC64_RS03070 (HFC64_03045) 609554..609778 - 225 WP_009993015.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HFC64_RS03075 (HFC64_03050) 610088..611003 - 916 Protein_622 IS110 family transposase -
HFC64_RS03080 (HFC64_03055) 611048..612038 + 991 Protein_623 IS630-like element ISC1048 family transposase -
HFC64_RS03085 (HFC64_03060) 612057..612179 + 123 Protein_624 IS110 family transposase -
HFC64_RS03090 (HFC64_03065) 612222..612683 - 462 WP_010923265.1 metal-sulfur cluster assembly factor -
HFC64_RS03095 (HFC64_03070) 612680..613810 - 1131 WP_009992604.1 aromatic/alkene monooxygenase hydroxylase subunit beta -
HFC64_RS03100 (HFC64_03075) 613822..614169 - 348 WP_009992603.1 MmoB/DmpM family protein -
HFC64_RS03105 (HFC64_03080) 614147..614551 - 405 WP_009992602.1 Rieske (2Fe-2S) protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside IScluster/Tn - - 606306..612038 5732


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(8-124)

Antitoxin

Post-segregation antitoxin CcdA

(5-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 15117.81 Da        Isoelectric Point: 9.3474

>T153431 WP_009993017.1 NZ_CP050869:c609564-609172 [Saccharolobus solfataricus]
MKDKEFLLDASALYSLLDYVDKVDVKKIHVLTLTFYEVGNVIWKEYYIHKKVKDPITLSRLFYKLMRKFNVIEDSPLEGV
MRIAIERGLTYYNASYAYVAESLGLILVSNDKELIRKANAISLKDLIKSM

Download         Length: 393 bp

>T153431 NZ_CP066905:c55695-55225 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8840.20 Da        Isoelectric Point: 5.0190

>AT153431 WP_009993015.1 NZ_CP050869:c609778-609554 [Saccharolobus solfataricus]
MSDVISVRVKKELKKKAEELGINVREVVEKALEEAIREKEKEELKDMTMKIKELMRDVSEYDWVSTVRESRDER

Download         Length: 225 bp

>AT153431 NZ_CP066905:c56135-55692 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E3GVD1

Antitoxin

Source ID Structure
AlphaFold DB A0A3G8ES43

References