Detailed information of TA system TA153418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3657477..3658204
• Replicon: chromosome
• Accession: NZ_CP050865
• Organism: Escherichia coli strain 8-3-Ti3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: HFC66_RS18260
• Protein ID: WP_000547563.1
• Coordinates: 3657477..3657788 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HFC66_RS18265
• Protein ID: WP_000126296.1
• Coordinates: 3657785..3658204 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFC66_RS18230	3652619..3654328	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
HFC66_RS18235	3654338..3654880	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
HFC66_RS18240	3654880..3655647	+	768	WP_073840462.1	formate hydrogenlyase subunit HycG	-
HFC66_RS18245	3655644..3656054	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
HFC66_RS18250	3656047..3656517	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
HFC66_RS18255	3656542..3657315	+	774	WP_001026444.1	hypothetical protein	-
HFC66_RS18260	3657477..3657788	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
HFC66_RS18265	3657785..3658204	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
HFC66_RS18270	3658318..3659706	-	1389	Protein_3552	6-phospho-beta-glucosidase AscB	-
HFC66_RS18275	3659749..3661214	-	1466	Protein_3553	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
HFC66_RS18280	3661464..3662474	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
HFC66_RS18285	3662623..3663150	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T153418 WP_000547563.1 NZ_CP050865:3657477-3657788 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T153418 NZ_CP066903:2792-3058 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT153418 WP_000126296.1 NZ_CP050865:3657785-3658204 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT153418 NZ_CP066903:2584-2808 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22