Detailed information of TA system TA153414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2859951..2860553
• Replicon: chromosome
• Accession: NZ_CP050865
• Organism: Escherichia coli strain 8-3-Ti3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HFC66_RS14395
• Protein ID: WP_000897305.1
• Coordinates: 2859951..2860262 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HFC66_RS14400
• Protein ID: WP_000356397.1
• Coordinates: 2860263..2860553 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFC66_RS14370	2856202..2857023	+	822	Protein_2798	hypothetical protein	-
HFC66_RS14375	2856909..2857181	-	273	WP_167839330.1	hypothetical protein	-
HFC66_RS14380	2857080..2857777	+	698	Protein_2800	IS1 family transposase	-
HFC66_RS14385	2857798..2858646	+	849	WP_167839485.1	transposase	-
HFC66_RS14390	2858814..2859722	-	909	WP_032360143.1	alpha/beta hydrolase	-
HFC66_RS14395	2859951..2860262	+	312	WP_000897305.1	hypothetical protein	Toxin
HFC66_RS14400	2860263..2860553	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HFC66_RS14405	2861139..2861357	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
HFC66_RS14410	2861508..2862205	+	698	Protein_2806	IS1 family transposase	-
HFC66_RS14415	2862353..2862595	+	243	WP_001086388.1	hypothetical protein	-
HFC66_RS14420	2862925..2863854	-	930	WP_032359289.1	formate dehydrogenase accessory protein FdhE	-
HFC66_RS14425	2863851..2864486	-	636	WP_073840738.1	formate dehydrogenase cytochrome b556 subunit	-
HFC66_RS14430	2864483..2865385	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2857274..2862205	4931

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T153414 WP_000897305.1 NZ_CP050865:2859951-2860262 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T153414 NZ_CP066900:c5361793-5361410 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT153414 WP_000356397.1 NZ_CP050865:2860263-2860553 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT153414 NZ_CP066900:c5362035-5361793 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7