Detailed information of TA system TA153412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2064541..2065340
• Replicon: chromosome
• Accession: NZ_CP050865
• Organism: Escherichia coli strain 8-3-Ti3

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A3I9P420
• Locus tag: HFC66_RS10405
• Protein ID: WP_000347271.1
• Coordinates: 2064876..2065340 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: HFC66_RS10400
• Protein ID: WP_001302819.1
• Coordinates: 2064541..2064876 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFC66_RS10385	2060326..2061096	-	771	WP_001058214.1	2-dehydro-3-deoxyglucarate aldolase	-
HFC66_RS10390	2061112..2062446	-	1335	WP_167839460.1	galactarate/glucarate/glycerate transporter GarP	-
HFC66_RS10395	2062821..2064392	+	1572	WP_001273770.1	galactarate dehydratase	-
HFC66_RS10400	2064541..2064876	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HFC66_RS10405	2064876..2065340	+	465	WP_000347271.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HFC66_RS10410	2065395..2066204	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HFC66_RS10415	2066453..2067733	+	1281	WP_032359524.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HFC66_RS10420	2067756..2068229	+	474	WP_016245132.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HFC66_RS10425	2068240..2069019	+	780	WP_000406215.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HFC66_RS10430	2069009..2069887	+	879	WP_001380079.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HFC66_RS10435	2069905..2070339	+	435	WP_000948814.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2054950..2065340	10390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17849.25 Da
• Isoelectric Point: 9.6924

>T153412 WP_000347271.1 NZ_CP050865:2064876-2065340 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEENH

Nucleotide

• Length: 465 bp

>T153412 NZ_CP066900:c4880371-4880087 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT153412 WP_001302819.1 NZ_CP050865:2064541-2064876 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT153412 NZ_CP066900:c4880603-4880361 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A3I9P420